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Myeloprotection with activated carbon in doxorubicin-treated rats.
Gerashchenko, Bogdan I; Sarnatskaya, Veronika V; Bardakhivska, Kvitoslava I; Sydorenko, Oleksii S; Kolesnik, Denis L; Klymchuk, Dmytro O.
Afiliación
  • Gerashchenko BI; R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology (IEPOR), National Academy of Sciences of Ukraine, Vasylkivska Str. 45, Kyiv, 03022, Ukraine.
  • Sarnatskaya VV; R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology (IEPOR), National Academy of Sciences of Ukraine, Vasylkivska Str. 45, Kyiv, 03022, Ukraine.
  • Bardakhivska KI; R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology (IEPOR), National Academy of Sciences of Ukraine, Vasylkivska Str. 45, Kyiv, 03022, Ukraine.
  • Sydorenko OS; R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology (IEPOR), National Academy of Sciences of Ukraine, Vasylkivska Str. 45, Kyiv, 03022, Ukraine.
  • Kolesnik DL; R.E. Kavetsky Institute of Experimental Pathology, Oncology and Radiobiology (IEPOR), National Academy of Sciences of Ukraine, Vasylkivska Str. 45, Kyiv, 03022, Ukraine.
  • Klymchuk DO; M.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Tereshchenkivska Str. 2, Kyiv, 01601, Ukraine.
Heliyon ; 9(7): e18414, 2023 Jul.
Article en En | MEDLINE | ID: mdl-37539240
Chemotherapy can often cause a variety of side effects including bone marrow (BM) suppression, termed as myelosuppression. Accordingly, facile and effective management of chemotherapy-induced myelosuppression is currently a pivotal task for experimental pathologists and oncologists. Here, we chose to use activated carbon (AC) with an extensive surface area for studying its possible protective effectiveness with respect to BM in doxorubicin (DOX)-treated rats. Spherical AC with an extended surface area up to 4490 m2/g was prepared for per os (p/o) delivery, whereas for intraperitoneal (i/p) delivery we used the powdered form of AC that was derived from the aforementioned spherical AC. During the monthly treatment of animals with AC and DOX these two components were delivered alternately (not in the same day). After treatment, BM cells were isolated from femurs of sacrificed animals, stained with acridine orange (AO) and analyzed by flow cytometry. Regardless of the route of AC delivery (p/o or i/p), apparent myeloprotection with a possible regenerative effect was observed in animals that received DOX, as evidenced by recovery of the populations of total nucleated cells (TNC) and polychromatic (immature) erythrocytes accompanied by a considerable reduction of the number of apoptotic/dead cells among TNC (≤2.0%). Moreover, as a result of AC administrations, there was a significant increase of AO green and far-red fluorescence intensities in the population of TNC, which is suggestive of the ongoing quantitative and conformational changes in DNA and RNA associated with cell recovery and proliferation. Thus, AC preparations under the present experimental conditions can effectively tackle DOX-induced myelosuppression via mechanisms not necessarily associated with adsorptive detoxification.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Heliyon Año: 2023 Tipo del documento: Article País de afiliación: Ucrania Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Heliyon Año: 2023 Tipo del documento: Article País de afiliación: Ucrania Pais de publicación: Reino Unido