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Dendritic Mesoporous Organosilica Nanoparticles with Photosensitizers for Cell Imaging, siRNA Delivery and Protein Loading.
Omar, Haneen; Jakimoska, Sara; Guillot, Julia; Alsharaeh, Edreese; Charnay, Clarence; Cunin, Frédérique; Bessière, Aurélie; Durand, Jean-Olivier; Raehm, Laurence; Lichon, Laure; Onofre, Mélanie; Gary-Bobo, Magali.
Afiliación
  • Omar H; Chemistry Department, Collage of Science, Alfaisal University, Riyadh 11533, Saudi Arabia.
  • Jakimoska S; IBMM, University Montpellier, CNRS, ENSCM, 34193 Montpellier, France.
  • Guillot J; ICGM, University Montpellier, CNRS, ENSCM, 34193 Montpellier, France.
  • Alsharaeh E; IBMM, University Montpellier, CNRS, ENSCM, 34193 Montpellier, France.
  • Charnay C; ICGM, University Montpellier, CNRS, ENSCM, 34193 Montpellier, France.
  • Cunin F; Chemistry Department, Collage of Science, Alfaisal University, Riyadh 11533, Saudi Arabia.
  • Bessière A; ICGM, University Montpellier, CNRS, ENSCM, 34193 Montpellier, France.
  • Durand JO; ICGM, University Montpellier, CNRS, ENSCM, 34193 Montpellier, France.
  • Raehm L; ICGM, University Montpellier, CNRS, ENSCM, 34193 Montpellier, France.
  • Lichon L; ICGM, University Montpellier, CNRS, ENSCM, 34193 Montpellier, France.
  • Onofre M; ICGM, University Montpellier, CNRS, ENSCM, 34193 Montpellier, France.
  • Gary-Bobo M; IBMM, University Montpellier, CNRS, ENSCM, 34193 Montpellier, France.
Molecules ; 28(14)2023 Jul 11.
Article en En | MEDLINE | ID: mdl-37513209
Dendritic mesoporous organosilica nanoparticles (DMON) are a new class of biodegradable nanoparticles suitable for biomolecule delivery. We studied the photochemical internalization (PCI) and photodynamic therapy (PDT) of DMON to investigate new ways for DMON to escape from the endosomes-lysosomes and deliver biomolecules into the cytoplasm of cells. We added photosensitizers in the framework of DMON and found that DMON were loaded with siRNA or FVIII factor protein. We made four formulations with four different photosensitizers. The photosensitizers allowed us to perform imaging of DMON in cancer cells, but the presence of the tetrasulfide bond in the framework of DMON quenched the formation of singlet oxygen. Fortunately, one formulation allowed us to efficiently deliver proapoptotic siRNA in MCF-7 cancer cells leading to 31% of cancer cell death, without irradiation. As for FVIII protein, it was loaded in two formulations with drug-loading capacities (DLC) up to 25%. In conclusion, DMON are versatile nanoparticles capable of loading siRNA and delivering it into cancer cells, and also loading FVIII protein with good DLC. Due to the presence of tetrasulfide, it was not possible to perform PDT or PCI.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fotoquimioterapia / Nanopartículas Límite: Humans Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Fotoquimioterapia / Nanopartículas Límite: Humans Idioma: En Revista: Molecules Asunto de la revista: BIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: Suiza