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Simultaneous degradation of aflatoxin B1 and zearalenone by Porin and Peroxiredoxin enzymes cloned from Acinetobacter nosocomialis Y1.
Adegoke, Tosin Victor; Yang, Bolei; Tian, Xiaoyu; Yang, Shuo; Gao, Yuan; Ma, Junning; Wang, Gang; Si, Peidong; Li, Runyan; Xing, Fuguo.
Afiliación
  • Adegoke TV; Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs / Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
  • Yang B; Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs / Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
  • Tian X; Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs / Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
  • Yang S; Feed Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
  • Gao Y; Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs / Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
  • Ma J; Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs / Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
  • Wang G; Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs / Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
  • Si P; Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs / Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
  • Li R; Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs / Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China.
  • Xing F; Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs / Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: xingfuguo@caas.cn.
J Hazard Mater ; 459: 132105, 2023 10 05.
Article en En | MEDLINE | ID: mdl-37494799
Mycotoxin contamination can cause severe health issues for both humans and animals. This study examined the potential of enzymes derived from Acinetobacter nosocomialis Y1 to simultaneously degrade aflatoxin B1 (AFB1) and zearalenone (ZEN), which could have significant implications in reducing mycotoxin contamination. Two enzymes, Porin and Peroxiredoxin, were identified with molecular weights of 27.8 and 20.8 kDa, respectively. Porin could completely degrade 2 µg/mL of AFB1 and ZEN within 24 h at 80 °C and 60 °C, respectively. Peroxiredoxin could completely degrade 2 µg/mL of AFB1 and reduce ZEN by 91.12% within 24 h. The addition of Na+, Cu2+, and K+ ions enhanced the degradation activities of both enzymes. LC-MS/MS analysis revealed that the molar masses of the degradation products of AFB1 and ZEN were 286 g/mol and 322.06 g/mol, and the products were identified as AFD1 and α or ß-ZAL, respectively. Vibrio fischeri bioluminescence assays further confirmed that the cytotoxicity of the two degradation products was significantly lower than that of AFB1 and ZEN. Based on these results, it can be inferred that the degradation product of ZEN is ß-ZAL. These findings suggest that both enzymes have the potential to be utilized as detoxification enzymes in food and feed.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Zearalenona / Micotoxinas Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: J Hazard Mater Asunto de la revista: SAUDE AMBIENTAL Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Zearalenona / Micotoxinas Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: J Hazard Mater Asunto de la revista: SAUDE AMBIENTAL Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Países Bajos