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Within-Host Rhinovirus Evolution in Upper and Lower Respiratory Tract Highlights Capsid Variability and Mutation-Independent Compartmentalization.
Makhsous, Negar; Goya, Stephanie; Avendaño, Carlos C; Rupp, Jason; Kuypers, Jane; Jerome, Keith R; Boeckh, Michael; Waghmare, Alpana; Greninger, Alexander L.
Afiliación
  • Makhsous N; Department of Laboratory Medicine and Pathology, University of Washington, Seattle, USA.
  • Goya S; Department of Laboratory Medicine and Pathology, University of Washington, Seattle, USA.
  • Avendaño CC; Department of Laboratory Medicine and Pathology, University of Washington, Seattle, USA.
  • Rupp J; Department of Laboratory Medicine and Pathology, University of Washington, Seattle, USA.
  • Kuypers J; Department of Laboratory Medicine and Pathology, University of Washington, Seattle, USA.
  • Jerome KR; Department of Laboratory Medicine and Pathology, University of Washington, Seattle, USA.
  • Boeckh M; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, USA.
  • Waghmare A; Vaccine and Infectious Disease Division, Fred Hutchinson Cancer Center, Seattle, USA.
  • Greninger AL; Department of Medicine, University of Washington, Seattle, USA.
J Infect Dis ; 229(2): 403-412, 2024 Feb 14.
Article en En | MEDLINE | ID: mdl-37486790
BACKGROUND: Rhinovirus (RV) infections can progress from the upper (URT) to lower (LRT) respiratory tract in immunocompromised individuals, causing high rates of fatal pneumonia. Little is known about how RV evolves within hosts during infection. METHODS: We sequenced RV complete genomes from 12 hematopoietic cell transplant patients with infection for up to 190 days from both URT (nasal wash, NW) and LRT (bronchoalveolar lavage, BAL). Metagenomic and amplicon next-generation sequencing were used to track the emergence and evolution of intrahost single nucleotide variants (iSNVs). RESULTS: Identical RV intrahost populations in matched NW and BAL specimens indicated no genetic adaptation is required for RV to progress from URT to LRT. Coding iSNVs were 2.3-fold more prevalent in capsid over nonstructural genes. iSNVs modeled were significantly more likely to be found in capsid surface residues, but were not preferentially located in known RV-neutralizing antibody epitopes. Newly emergent, genotype-matched iSNV haplotypes from immunocompromised individuals in 2008-2010 could be detected in Seattle-area community RV sequences in 2020-2021. CONCLUSIONS: RV infections in immunocompromised hosts can progress from URT to LRT with no specific evolutionary requirement. Capsid proteins carry the highest variability and emergent mutations can be detected in other, including future, RV sequences.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trasplante de Células Madre Hematopoyéticas / Infecciones por Enterovirus Límite: Humans Idioma: En Revista: J Infect Dis Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trasplante de Células Madre Hematopoyéticas / Infecciones por Enterovirus Límite: Humans Idioma: En Revista: J Infect Dis Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos