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Subcellular positioning during cell division and cell plate formation in maize.
Allsman, Lindy A; Bellinger, Marschal A; Huang, Vivian; Duong, Matthew; Contreras, Alondra; Romero, Andrea N; Verboonen, Benjamin; Sidhu, Sukhmani; Zhang, Xiaoguo; Steinkraus, Holly; Uyehara, Aimee N; Martinez, Stephanie E; Sinclair, Rosalie M; Soriano, Gabriela Salazar; Diep, Beatrice; Byrd V, Dawson; Noriega, Alexander; Drakakaki, Georgia; Sylvester, Anne W; Rasmussen, Carolyn G.
Afiliación
  • Allsman LA; Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, Riverside, CA, United States.
  • Bellinger MA; Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, Riverside, CA, United States.
  • Huang V; Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, Riverside, CA, United States.
  • Duong M; Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, Riverside, CA, United States.
  • Contreras A; Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, Riverside, CA, United States.
  • Romero AN; Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, Riverside, CA, United States.
  • Verboonen B; Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, Riverside, CA, United States.
  • Sidhu S; Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, Riverside, CA, United States.
  • Zhang X; Department of Molecular Biology, University of Wyoming, Laramie, WY, United States.
  • Steinkraus H; Department of Molecular Biology, University of Wyoming, Laramie, WY, United States.
  • Uyehara AN; Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, Riverside, CA, United States.
  • Martinez SE; Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, Riverside, CA, United States.
  • Sinclair RM; Department of Plant Sciences, University of California, Davis, Davis, CA, United States.
  • Soriano GS; Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, Riverside, CA, United States.
  • Diep B; Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, Riverside, CA, United States.
  • Byrd V D; Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, Riverside, CA, United States.
  • Noriega A; Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, Riverside, CA, United States.
  • Drakakaki G; Department of Plant Sciences, University of California, Davis, Davis, CA, United States.
  • Sylvester AW; Department of Molecular Biology, University of Wyoming, Laramie, WY, United States.
  • Rasmussen CG; Department of Botany and Plant Sciences, Center for Plant Cell Biology, University of California, Riverside, Riverside, CA, United States.
Front Plant Sci ; 14: 1204889, 2023.
Article en En | MEDLINE | ID: mdl-37484472
Introduction: During proliferative plant cell division, the new cell wall, called the cell plate, is first built in the middle of the cell and then expands outward to complete cytokinesis. This dynamic process requires coordinated movement and arrangement of the cytoskeleton and organelles. Methods: Here we use live-cell markers to track the dynamic reorganization of microtubules, nuclei, endoplasmic reticulum, and endomembrane compartments during division and the formation of the cell plate in maize leaf epidermal cells. Results: The microtubule plus-end localized protein END BINDING1 (EB1) highlighted increasing microtubule dynamicity during mitosis to support rapid changes in microtubule structures. The localization of the cell-plate specific syntaxin KNOLLE, several RAB-GTPases, as well as two plasma membrane localized proteins was assessed after treatment with the cytokinesis-specific callose-deposition inhibitor Endosidin7 (ES7) and the microtubule-disrupting herbicide chlorpropham (CIPC). While ES7 caused cell plate defects in Arabidopsis thaliana, it did not alter callose accumulation, or disrupt cell plate formation in maize. In contrast, CIPC treatment of maize epidermal cells occasionally produced irregular cell plates that split or fragmented, but did not otherwise disrupt the accumulation of cell-plate localized proteins. Discussion: Together, these markers provide a robust suite of tools to examine subcellular trafficking and organellar organization during mitosis and cell plate formation in maize.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Plant Sci Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Plant Sci Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza