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Microbiota perturbation by anti-microbiota vaccine reduces the colonization of Borrelia afzelii in Ixodes ricinus.
Wu-Chuang, Alejandra; Mateos-Hernandez, Lourdes; Maitre, Apolline; Rego, Ryan O M; Síma, Radek; Porcelli, Stefania; Rakotobe, Sabine; Foucault-Simonin, Angélique; Moutailler, Sara; Palinauskas, Vaidas; Azelyte, Juste; Sǐmo, Ladislav; Obregon, Dasiel; Cabezas-Cruz, Alejandro.
Afiliación
  • Wu-Chuang A; Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, 94700, Maisons-Alfort, France.
  • Mateos-Hernandez L; Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, 94700, Maisons-Alfort, France.
  • Maitre A; Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, 94700, Maisons-Alfort, France.
  • Rego ROM; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, Ceske Budejovice, Czech Republic.
  • Síma R; Faculty of Science, University of South Bohemia, Ceske Budejovice, Czech Republic.
  • Porcelli S; Institute of Parasitology, Biology Centre, Czech Academy of Sciences, Ceske Budejovice, Czech Republic.
  • Rakotobe S; Biopticka Laborator S.R.O, Plzen, Czech Republic.
  • Foucault-Simonin A; Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, 94700, Maisons-Alfort, France.
  • Moutailler S; Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, 94700, Maisons-Alfort, France.
  • Palinauskas V; Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, 94700, Maisons-Alfort, France.
  • Azelyte J; Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, 94700, Maisons-Alfort, France.
  • Sǐmo L; Nature Research Centre, Akademijos 2, 09412, Vilnius, Lithuania.
  • Obregon D; Nature Research Centre, Akademijos 2, 09412, Vilnius, Lithuania.
  • Cabezas-Cruz A; Anses, INRAE, Ecole Nationale Vétérinaire d'Alfort, UMR BIPAR, Laboratoire de Santé Animale, 94700, Maisons-Alfort, France.
Microbiome ; 11(1): 151, 2023 07 24.
Article en En | MEDLINE | ID: mdl-37482606
BACKGROUND: Ticks can transmit a broad variety of pathogens of medical importance, including Borrelia afzelii, the causative agent of Lyme borreliosis in Europe. Tick microbiota is an important factor modulating, not only vector physiology, but also the vector competence. Anti-microbiota vaccines targeting keystone taxa of tick microbiota can alter tick feeding and modulate the taxonomic and functional profiles of bacterial communities in the vector. However, the impact of anti-microbiota vaccine on tick-borne pathogen development within the vector has not been tested. RESULTS: Here, we characterized the Ixodes ricinus microbiota modulation in response to B. afzelii infection and found that the pathogen induces changes in the microbiota composition, its beta diversity and structure of bacterial community assembly. Tick microbiota perturbation by anti-microbiota antibodies or addition of novel commensal bacteria into tick midguts causes departures from the B. afzelii-induced modulation of tick microbiota which resulted in a lower load of the pathogen in I. ricinus. Co-occurrence networks allowed the identification of emergent properties of the bacterial communities which better defined the Borrelia infection-refractory states of the tick microbiota. CONCLUSIONS: These findings suggest that Borrelia is highly sensitive to tick microbiota perturbations and that departure from the modulation induced by the pathogen in the vector microbiota pose a high cost to the spirochete. Network analysis emerges as a suitable tool to identify emergent properties of the vector microbiota associated with infection-refractory states. Anti-microbiota vaccines can be used as a tool for microbiota perturbation and control of important vector-borne pathogens. Video Abstract.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedad de Lyme / Grupo Borrelia Burgdorferi / Ixodes Tipo de estudio: Prognostic_studies Límite: Animals País/Región como asunto: Europa Idioma: En Revista: Microbiome Año: 2023 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedad de Lyme / Grupo Borrelia Burgdorferi / Ixodes Tipo de estudio: Prognostic_studies Límite: Animals País/Región como asunto: Europa Idioma: En Revista: Microbiome Año: 2023 Tipo del documento: Article País de afiliación: Francia Pais de publicación: Reino Unido