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Hemicyanine-based sensor for mitochondrial viscosity imaging in BV2 cells.
Ma, Sufang; Zhou, Liang; Ma, Yingyu; Zhao, Huanhuan; Li, Leyan; Wang, Meiling; Diao, Haipeng; Li, Xiaowan; Zhang, Chengwu; Liu, Wen.
Afiliación
  • Ma S; College of Basic Medical Science, Shanxi Medical University, Taiyuan 030001, PR China.
  • Zhou L; College of Basic Medical Science, Shanxi Medical University, Taiyuan 030001, PR China.
  • Ma Y; College of Basic Medical Science, Shanxi Medical University, Taiyuan 030001, PR China.
  • Zhao H; College of Basic Medical Science, Shanxi Medical University, Taiyuan 030001, PR China.
  • Li L; College of Basic Medical Science, Shanxi Medical University, Taiyuan 030001, PR China.
  • Wang M; College of Basic Medical Science, Shanxi Medical University, Taiyuan 030001, PR China.
  • Diao H; College of Basic Medical Science, Shanxi Medical University, Taiyuan 030001, PR China; Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University, Taiyuan 030001, PR China. Electronic address: diaohp@sxmu.edu.cn.
  • Li X; College of Basic Medical Science, Shanxi Medical University, Taiyuan 030001, PR China.
  • Zhang C; College of Basic Medical Science, Shanxi Medical University, Taiyuan 030001, PR China. Electronic address: chengwu_zhang@sxmu.edu.cn.
  • Liu W; College of Basic Medical Science, Shanxi Medical University, Taiyuan 030001, PR China; Key Laboratory of Cellular Physiology, Ministry of Education, Shanxi Medical University, Taiyuan 030001, PR China. Electronic address: liuwen@sxmu.edu.cn.
Spectrochim Acta A Mol Biomol Spectrosc ; 302: 123132, 2023 Dec 05.
Article en En | MEDLINE | ID: mdl-37478757
Mitochondrial viscosity is a critical factor affecting numerous physiological processes, including phagocytosis. Abnormal viscosity in mitochondria is related to some pathological activities and diseases. Evaluating and detecting the changes in mitochondrial viscosity in vivo is crucial. Thus, a mitochondria-targeted red-emitting fluorescent probe (VP) was prepared, and can be used to detect viscosity with high selectivity and sensitivity. The synthesis of probe VP was as simple as two steps and the cost was low. In addition, the fluorescence intensity (log I615) exhibited an excellent relationship with viscosity (log η) in the range of 0.5 - 2.5 (R2 = 0.9985) in water/glycerol mixture. It is noteworthy that the probe VP displayed the highest signal-to-noise ratio (about 50-fold) for viscosity in water and glycerol system. The probe VP can visualize the mitochondrial viscosity change in living cells. More importantly, phagocytic test for BV2 cells further demonstrated that phagocytosis decreased with increased viscosity. Furthermore, VP was successfully used for monitoring the mitophagy process induced by starvation, and mitochondrial viscosity exhibited enhancement during mitophagy. The probe was a potential tool for studying viscosity and phagocytosis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Imagen Óptica / Glicerol Límite: Humans Idioma: En Revista: Spectrochim Acta A Mol Biomol Spectrosc Asunto de la revista: BIOLOGIA MOLECULAR Año: 2023 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Imagen Óptica / Glicerol Límite: Humans Idioma: En Revista: Spectrochim Acta A Mol Biomol Spectrosc Asunto de la revista: BIOLOGIA MOLECULAR Año: 2023 Tipo del documento: Article Pais de publicación: Reino Unido