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Miniaturized microarray-format digital ELISA enabled by lithographic protein patterning.
Stephens, Andrew D; Song, Yujing; McClellan, Brandon L; Su, Shiuan-Haur; Xu, Sonnet; Chen, Kevin; Castro, Maria G; Singer, Benjamin H; Kurabayashi, Katsuo.
Afiliación
  • Stephens AD; Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.
  • Song Y; Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.
  • McClellan BL; Department of Neurosurgery, University of Michigan Medical School, Ann Arbor, MI, 48109, USA; Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, 48109, USA; Graduate Program in Immunology, University of Michigan Medical School, Ann Arbor, MI, 48109, U
  • Su SH; Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.
  • Xu S; Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.
  • Chen K; Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.
  • Castro MG; Department of Neurosurgery, University of Michigan Medical School, Ann Arbor, MI, 48109, USA; Department of Cell and Developmental Biology, University of Michigan Medical School, Ann Arbor, MI, 48109, USA; Rogel Cancer Center, University of Michigan, Ann Arbor, MI, 48109, USA.
  • Singer BH; Department of Internal Medicine, Division of Pulmonary and Critical Care Medicine, University of Michigan, Ann Arbor, MI, 48109, USA; Weil Institute for Critical Care Research and Innovation, University of Michigan, Ann Arbor, MI, 48109, USA.
  • Kurabayashi K; Department of Mechanical and Aerospace Engineering, New York University, Brooklyn, NY, 11201, USA. Electronic address: kk5165@nyu.edu.
Biosens Bioelectron ; 237: 115536, 2023 Oct 01.
Article en En | MEDLINE | ID: mdl-37473549
The search for reliable protein biomarker candidates is critical for early disease detection and treatment. However, current immunoassay technologies are failing to meet increasing demands for sensitivity and multiplexing. Here, the authors have created a highly sensitive protein microarray using the principle of single-molecule counting for signal amplification, capable of simultaneously detecting a panel of cancer biomarkers at sub-pg/mL levels. To enable this amplification strategy, the authors introduce a novel method of protein patterning using photolithography to subdivide addressable arrays of capture antibody spots into hundreds of thousands of individual microwells. This allows for the total sensor area to be miniaturized, increasing the total possible multiplex capacity. With the immunoassay realized on a standard 75x25 mm form factor glass substrate, sample volume consumption is minimized to <10 µL, making the technology highly efficient and cost-effective. Additionally, the authors demonstrate the power of their technology by measuring six secretory factors related to glioma tumor progression in a cohort of mice. This highly sensitive, sample-sparing multiplex immunoassay paves the way for researchers to track changes in protein profiles over time, leading to earlier disease detection and discovery of more effective treatment using animal models.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas Biosensibles Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido