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A Single-Molecule Bioelectronic Portable Array for Early Diagnosis of Pancreatic Cancer Precursors.
Genco, Enrico; Modena, Francesco; Sarcina, Lucia; Björkström, Kim; Brunetti, Celestino; Caironi, Mario; Caputo, Mariapia; Demartis, Virginia Maria; Di Franco, Cinzia; Frusconi, Giulia; Haeberle, Lena; Larizza, Piero; Mancini, Maria Teresa; Österbacka, Ronald; Reeves, William; Scamarcio, Gaetano; Scandurra, Cecilia; Wheeler, May; Cantatore, Eugenio; Esposito, Irene; Macchia, Eleonora; Torricelli, Fabrizio; Viola, Fabrizio Antonio; Torsi, Luisa.
Afiliación
  • Genco E; Department of Electrical Engineering, Eindhoven University of Technology, Eindhoven, 5600 MB, The Netherlands.
  • Modena F; Center for Nano Science and Technology, Istituto Italiano di Tecnologia, Via Rubattino 81, Milan, 20134, Italy.
  • Sarcina L; Dipartimento di Elettronica, Informazione e Bioingegneria, Politecnico di Milano, Piazza Leonardo da Vinci 32, Milan, 20133, Italy.
  • Björkström K; Dipartimento di Chimica and Centre for Colloid and Surface Science, Università degli Studi di Bari Aldo Moro, Bari, 20125, Italy.
  • Brunetti C; The Faculty of Science and Engineering, Åbo Akademi University, Turku, 20500, Finland.
  • Caironi M; Masmec Biomed - Masmec SpA division, Modugno (BA), 70026, Italy.
  • Caputo M; Center for Nano Science and Technology, Istituto Italiano di Tecnologia, Via Rubattino 81, Milan, 20134, Italy.
  • Demartis VM; Dipartimento di Farmacia-Scienze del Farmaco, Università degli Studi di Bari "Aldo Moro", Bari, 70125, Italy.
  • Di Franco C; Dipartimento Ingegneria dell'Informazione, Università degli Studi di Brescia, Brescia, 25123, Italy.
  • Frusconi G; CNR IFN, Bari, 70126, Italy.
  • Haeberle L; Dipartimento Ingegneria dell'Informazione, Università degli Studi di Brescia, Brescia, 25123, Italy.
  • Larizza P; Institute of Pathology, Heinrich-Heine University and University Hospital of Düsseldorf, 40225, Duesseldorf, Germany.
  • Mancini MT; Masmec Biomed - Masmec SpA division, Modugno (BA), 70026, Italy.
  • Österbacka R; Masmec Biomed - Masmec SpA division, Modugno (BA), 70026, Italy.
  • Reeves W; The Faculty of Science and Engineering, Åbo Akademi University, Turku, 20500, Finland.
  • Scamarcio G; FlexEnable Technology Ltd, Cambridge, CB4 0FX, UK.
  • Scandurra C; CNR IFN, Bari, 70126, Italy.
  • Wheeler M; Dipartimento Interateneo di Fisica, Università degli Studi di Bari Aldo Moro, Bari, 70125, Italy.
  • Cantatore E; Dipartimento di Chimica and Centre for Colloid and Surface Science, Università degli Studi di Bari Aldo Moro, Bari, 20125, Italy.
  • Esposito I; FlexEnable Technology Ltd, Cambridge, CB4 0FX, UK.
  • Macchia E; Department of Electrical Engineering, Eindhoven University of Technology, Eindhoven, 5600 MB, The Netherlands.
  • Torricelli F; Institute of Pathology, Heinrich-Heine University and University Hospital of Düsseldorf, 40225, Duesseldorf, Germany.
  • Viola FA; The Faculty of Science and Engineering, Åbo Akademi University, Turku, 20500, Finland.
  • Torsi L; Dipartimento di Farmacia-Scienze del Farmaco, Università degli Studi di Bari "Aldo Moro", Bari, 70125, Italy.
Adv Mater ; 35(42): e2304102, 2023 Oct.
Article en En | MEDLINE | ID: mdl-37452695
A cohort of 47 patients is screened for pancreatic cancer precursors with a portable 96-well bioelectronic sensing-array for single-molecule assay in cysts fluid and blood plasma, deployable at point-of-care (POC). Pancreatic cancer precursors are mucinous cysts diagnosed with a sensitivity of at most 80% by state-of-the-art cytopathological molecular analyses (e.g., KRASmut DNA). Adding the simultaneous assay of proteins related to malignant transformation (e.g., MUC1 and CD55) is deemed essential to enhance diagnostic accuracy. The bioelectronic array proposed here, based on single-molecule-with-a-large-transistor (SiMoT) technology, can assay both nucleic acids and proteins at the single-molecule limit-of-identification (LOI) (1% of false-positives and false-negatives). It comprises an enzyme-linked immunosorbent assay (ELISA)-like 8 × 12-array organic-electronics disposable cartridge with an electrolyte-gated organic transistor sensor array, and a reusable reader, integrating a custom Si-IC chip, operating via software installed on a USB-connected smart device. The cartridge is complemented by a 3D-printed sensing gate cover plate. KRASmut , MUC1, and CD55 biomarkers either in plasma or cysts-fluid from 5 to 6 patients at a time, are multiplexed at single-molecule LOI in 1.5 h. The pancreatic cancer precursors are classified via a machine-learning analysis resulting in at least 96% diagnostic-sensitivity and 100% diagnostic-specificity. This preliminary study opens the way to POC liquid-biopsy-based early diagnosis of pancreatic-cancer precursors in plasma.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Pancreáticas / Quistes Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Humans Idioma: En Revista: Adv Mater Asunto de la revista: BIOFISICA / QUIMICA Año: 2023 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias Pancreáticas / Quistes Tipo de estudio: Diagnostic_studies / Screening_studies Límite: Humans Idioma: En Revista: Adv Mater Asunto de la revista: BIOFISICA / QUIMICA Año: 2023 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Alemania