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Detection of HPV in urine for cervical cancer screening: Feasibilty of an assay system.
Li, Guorong; Moniod, Louise; Chenafi, Sara; Lamsisi, Maryame; Ennaji, Moulay Mustapha; Bourlet, Thomas; Chauleur, Céline.
Afiliación
  • Li G; Department of Urology, North Hospital, CHU Saint-Etienne, Saint-Etienne, France. Electronic address: guorong.li@chu-st-etienne.fr.
  • Moniod L; Department of Gynecology and Obstetrics, North Hospital, CHU Saint-Etienne, Saint-Etienne, France.
  • Chenafi S; Laboratoire des Agents Infectieux et d'Hygiène, North Hospital, CHU Saint-Etienne, Saint-Etienne, France.
  • Lamsisi M; Team of Virology, Oncology and Biotechnologies, Laboratory of Virology, Oncology, Biosciences, Environment and New Energies. Faculty of Sciences and Techniques Mohammedia, University Hassan II of Casablanca, Morocco.
  • Ennaji MM; Team of Virology, Oncology and Biotechnologies, Laboratory of Virology, Oncology, Biosciences, Environment and New Energies. Faculty of Sciences and Techniques Mohammedia, University Hassan II of Casablanca, Morocco.
  • Bourlet T; Laboratoire des Agents Infectieux et d'Hygiène, North Hospital, CHU Saint-Etienne, Saint-Etienne, France; GIMAP Team 15, Centre International de Recherche en Infectiologie, Inserm, U1111, CNRS, UMR5308, University of Saint-Etienne, University of Lyon, France.
  • Chauleur C; Department of Gynecology and Obstetrics, North Hospital, CHU Saint-Etienne, Saint-Etienne, France; Inserm, SAINBIOSE, U1059, Université Jean-Monnet, CIC1408 F- 42055, Saint-Etienne, France.
J Gynecol Obstet Hum Reprod ; 52(8): 102631, 2023 Oct.
Article en En | MEDLINE | ID: mdl-37451412
The detection of urine HPV is considered as a promosing alternative to increase the screening coverage of cervical cancer. However, the validated assay of urine HPV is still scarse. We described a nouvel assay syetem for the urine-based detection of HPV in the framework of HPV screening. This sytsem consisted of Automate Nimbus extraction of DNA and Anyplex™ II HPV HR Detection PCR of HPV DNA. We validated this system by spiking HPV-infected cervical cancer cell line HeLa cells into normal urine and compared the prelimary results of cervical samples and urine samples. We found that this system could detect as few as 5 HeLa cells in normal urine model. Some discordances of HPV results between cervical samples and urine samples were observed. We concluded that this assay system could be applied for the detection of HPV in urine. A large scale study is necessary to evaluate the clinical significance of this assay system.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias del Cuello Uterino / Infecciones por Papillomavirus Tipo de estudio: Diagnostic_studies / Prognostic_studies / Screening_studies Límite: Female / Humans Idioma: En Revista: J Gynecol Obstet Hum Reprod Año: 2023 Tipo del documento: Article Pais de publicación: Francia
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias del Cuello Uterino / Infecciones por Papillomavirus Tipo de estudio: Diagnostic_studies / Prognostic_studies / Screening_studies Límite: Female / Humans Idioma: En Revista: J Gynecol Obstet Hum Reprod Año: 2023 Tipo del documento: Article Pais de publicación: Francia