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Effects of Individual Circulating FFAs on Plasma and Hepatic FFA Epoxides, Diols, and Epoxide-Diol Ratios as Indices of Soluble Epoxide Hydrolase Activity.
Oh, Young Taek; Yang, Jun; Morisseau, Christophe; He, Qiyi; Hammock, Bruce; Youn, Jang H.
Afiliación
  • Oh YT; Department of Physiology and Neuroscience, University of Southern California Keck School of Medicine, 2250 Alcazar Street, CSC 214, Los Angeles, CA 90089, USA.
  • Yang J; Department of Entomology and Nematology, University of California, Davis, CA 95616, USA.
  • Morisseau C; Department of Entomology and Nematology, University of California, Davis, CA 95616, USA.
  • He Q; Department of Entomology and Nematology, University of California, Davis, CA 95616, USA.
  • Hammock B; Department of Entomology and Nematology, University of California, Davis, CA 95616, USA.
  • Youn JH; Department of Physiology and Neuroscience, University of Southern California Keck School of Medicine, 2250 Alcazar Street, CSC 214, Los Angeles, CA 90089, USA.
Int J Mol Sci ; 24(13)2023 Jun 28.
Article en En | MEDLINE | ID: mdl-37445935
Oxylipins, oxidation products of unsaturated free fatty acids (FFAs), are involved in various cellular signaling systems. Among these oxylipins, FFA epoxides are associated with beneficial effects in metabolic and cardiovascular health. FFA epoxides are metabolized to diols, which are usually biologically less active, by soluble epoxide hydrolase (sEH). Plasma epoxide-diol ratios have been used as indirect measures of sEH activity. This study was designed to examine the effects of acute elevation of individual plasma FFAs on a variety of oxylipins, particularly epoxides, diols, and their ratios. We tested if FFA epoxide-diol ratios are altered by circulating FFA levels (i.e., substrate availability) independent of sEH activity. Wistar rats received a constant intravenous infusion of olive (70% oleic acid (OA)), safflower seed (72% linoleic acid (LA)), and fish oils (rich in ω-3 FFAs) as emulsions to selectively raise OA, LA, and ω-3 FFAs (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)), respectively. As expected, olive, safflower seed, and fish oil infusions selectively raised plasma OA (57%), LA (87%), EPA (70%), and DHA (54%), respectively (p < 0.05 for all). Raising plasma FFAs exerted substrate effects to increase hepatic and plasma epoxide and diol levels. These increases in epoxides and diols occurred to similar extents, resulting in no significant changes in epoxide-diol ratios. These data suggest that epoxide-diol ratios, often used as indices of sEH activity, are not affected by substrate availability or altered plasma FFA levels and that epoxide-diol ratios may be used to compare sEH activity between conditions of different circulating FFA levels.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxilipinas / Ácidos Grasos no Esterificados Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza
Texto completo
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Imprimir
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oxilipinas / Ácidos Grasos no Esterificados Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Int J Mol Sci Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza