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Co-Expression of Immunohistochemical Markers MRP2, CXCR4, and PD-L1 in Gallbladder Tumors Is Associated with Prolonged Patient Survival.
Tittarelli, Andrés; Barría, Omar; Sanders, Evy; Bergqvist, Anna; Brange, Daniel Uribe; Vidal, Mabel; Gleisner, María Alejandra; Vergara, Jorge Ramón; Niechi, Ignacio; Flores, Iván; Pereda, Cristián; Carrasco, Cristian; Quezada-Monrás, Claudia; Salazar-Onfray, Flavio.
Afiliación
  • Tittarelli A; Programa Institucional de Fomento a la Investigación, Desarrollo e Innovación, Universidad Tecnológica Metropolitana, Santiago 8940577, Chile.
  • Barría O; Millennium Institute on Immunology and Immunotherapy, Santiago 8380453, Chile.
  • Sanders E; Disciplinary Program of Immunology, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile, Santiago 8380453, Chile.
  • Bergqvist A; Millennium Institute on Immunology and Immunotherapy, Santiago 8380453, Chile.
  • Brange DU; Disciplinary Program of Immunology, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile, Santiago 8380453, Chile.
  • Vidal M; Millennium Institute on Immunology and Immunotherapy, Santiago 8380453, Chile.
  • Gleisner MA; Disciplinary Program of Immunology, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile, Santiago 8380453, Chile.
  • Vergara JR; Laboratorio de Biología Tumoral, Instituto de Bioquímica y Microbiología, Universidad Austral de Chile, Valdivia 5090000, Chile.
  • Niechi I; Molecular and Traslational Immunology Laboratory, Department of Clinical Biochemistry and Immunology, Pharmacy Faculty, Universidad de Concepción, Concepción 4070386, Chile.
  • Flores I; Computer Science Department, Universidad de Concepción, Concepción 4070386, Chile.
  • Pereda C; Millennium Institute on Immunology and Immunotherapy, Santiago 8380453, Chile.
  • Carrasco C; Disciplinary Program of Immunology, Institute of Biomedical Sciences, Faculty of Medicine, Universidad de Chile, Santiago 8380453, Chile.
  • Quezada-Monrás C; Departamento de Informática y Computación, Universidad Tecnológica Metropolitana, Santiago 7800002, Chile.
  • Salazar-Onfray F; Laboratorio de Biología Tumoral, Instituto de Bioquímica y Microbiología, Universidad Austral de Chile, Valdivia 5090000, Chile.
Cancers (Basel) ; 15(13)2023 Jun 30.
Article en En | MEDLINE | ID: mdl-37444550
Gallbladder cancer (GBC) is a rare pathology in Western countries. However, it constitutes a relevant health problem in Asia and Latin America, with a high mortality in middle-aged Chilean women. The limited therapeutic options for GBC require the identification of targetable proteins with prognostic value for improving clinical management support. We evaluated the expression of targetable proteins, including three epithelial tumor markers, four proteins associated with multidrug and apoptosis resistance, and eleven immunological markers in 241 primary gallbladder adenocarcinomas. We investigated correlations between tumor marker expression, the primary tumor staging, and GBC patients' survival using automated immunohistochemistry, a semi-automatic method for image analysis, univariate and multivariate statistical analyses, and machine learning algorithms. Our data show a significant association between the expression of MRP2 (p = 0.0028), CXCR4 (p = 0.0423), and PD-L1 (p = 0.0264), and a better prognosis for patients with late-stage primary tumors. The expression of the MRP2/CXCR4/PD-L1 cluster of markers discriminates among short-, medium-, and long-term patient survival, with an ROC of significant prognostic value (AUC = 0.85, p = 0.0012). Moreover, a high MRP2/CXCR4/PD-L1 co-expression is associated with increased survival time (30 vs. 6 months, p = 0.0025) in GBC patients, regardless of tumor stage. Hence, our results suggest that the MRP2/CXCR4/PD-L1 cluster could potentially be a prognostic marker for GBC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Risk_factors_studies Idioma: En Revista: Cancers (Basel) Año: 2023 Tipo del documento: Article País de afiliación: Chile Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Risk_factors_studies Idioma: En Revista: Cancers (Basel) Año: 2023 Tipo del documento: Article País de afiliación: Chile Pais de publicación: Suiza