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Ex Vivo Preservation of Ovine Periosteum Using a Perfusion Bioreactor System.
Xin, Hai; Romanazzo, Sara; Tomaskovic-Crook, Eva; Mitchell, Timothy C; Hung, Jui Chien; Wise, Steven G; Cheng, Kai; Al Maruf, D S Abdullah; Stokan, Murray J; Manzie, Timothy G H; Parthasarathi, Krishnan; Cheung, Veronica K Y; Gupta, Ruta; Ly, Mark; Pulitano, Carlo; Wise, Innes K; Crook, Jeremy M; Clark, Jonathan R.
Afiliación
  • Xin H; Integrated Prosthetics and Reconstruction, Department of Head and Neck Surgery, Chris O'Brien Lifehouse, Camperdown, NSW 2050, Australia.
  • Romanazzo S; Central Clinical School, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2050, Australia.
  • Tomaskovic-Crook E; Arto Hardy Family Biomedical Innovation Hub, Chris O'Brien Lifehouse, Camperdown, NSW 2050, Australia.
  • Mitchell TC; Arto Hardy Family Biomedical Innovation Hub, Chris O'Brien Lifehouse, Camperdown, NSW 2050, Australia.
  • Hung JC; School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.
  • Wise SG; Intelligent Polymer Research Institute, University of Wollongong, Innovation Campus, Squires Way, North Wollongong, NSW 2500, Australia.
  • Cheng K; School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.
  • Al Maruf DSA; School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.
  • Stokan MJ; School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Sydney, NSW 2006, Australia.
  • Manzie TGH; Integrated Prosthetics and Reconstruction, Department of Head and Neck Surgery, Chris O'Brien Lifehouse, Camperdown, NSW 2050, Australia.
  • Parthasarathi K; Royal Prince Alfred Institute of Academic Surgery, Royal Prince Alfred Hospital, Sydney Local Health District, Camperdown, NSW 2050, Australia.
  • Cheung VKY; Integrated Prosthetics and Reconstruction, Department of Head and Neck Surgery, Chris O'Brien Lifehouse, Camperdown, NSW 2050, Australia.
  • Gupta R; Central Clinical School, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2050, Australia.
  • Ly M; Integrated Prosthetics and Reconstruction, Department of Head and Neck Surgery, Chris O'Brien Lifehouse, Camperdown, NSW 2050, Australia.
  • Pulitano C; Integrated Prosthetics and Reconstruction, Department of Head and Neck Surgery, Chris O'Brien Lifehouse, Camperdown, NSW 2050, Australia.
  • Wise IK; Integrated Prosthetics and Reconstruction, Department of Head and Neck Surgery, Chris O'Brien Lifehouse, Camperdown, NSW 2050, Australia.
  • Crook JM; Central Clinical School, Faculty of Medicine and Health, The University of Sydney, Camperdown, NSW 2050, Australia.
  • Clark JR; The Department of Tissue Pathology and Diagnostic Oncology, Royal Prince Alfred Hospital, Camperdown, NSW 2050, Australia.
Cells ; 12(13)2023 06 27.
Article en En | MEDLINE | ID: mdl-37443758
Periosteum is a highly vascularized membrane lining the surface of bones. It plays essential roles in bone repair following injury and reconstruction following invasive surgeries. To broaden the use of periosteum, including for augmenting in vitro bone engineering and/or in vivo bone repair, we have developed an ex vivo perfusion bioreactor system to maintain the cellular viability and metabolism of surgically resected periosteal flaps. Each specimen was placed in a 3D printed bioreactor connected to a peristaltic pump designed for the optimal flow rates of tissue perfusate. Nutrients and oxygen were perfused via the periosteal arteries to mimic physiological conditions. Biochemical assays and histological staining indicate component cell viability after perfusion for almost 4 weeks. Our work provides the proof-of-concept of ex vivo periosteum perfusion for long-term tissue preservation, paving the way for innovative bone engineering approaches that use autotransplanted periosteum to enhance in vivo bone repair.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Periostio / Ingeniería de Tejidos Límite: Animals Idioma: En Revista: Cells Año: 2023 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Periostio / Ingeniería de Tejidos Límite: Animals Idioma: En Revista: Cells Año: 2023 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Suiza