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Prediction of Distant Metastases in Patients with Kidney Cancer Based on Gene Expression and Methylation Analysis.
Apanovich, Natalya; Matveev, Alexey; Ivanova, Natalia; Burdennyy, Alexey; Apanovich, Pavel; Pronina, Irina; Filippova, Elena; Kazubskaya, Tatiana; Loginov, Vitaly; Braga, Eleonora; Alimov, Andrei.
Afiliación
  • Apanovich N; Research Centre for Medical Genetics, 1 Moskvorechye St., Moscow 115522, Russia.
  • Matveev A; Federal State Budgetary Institution (N.N. Blokhin National Medical Research Center of Oncology) of the Ministry of Health of the Russian Federation, 24 Kashirskoe Shosse, Moscow 115478, Russia.
  • Ivanova N; Institute of General Pathology and Pathophysiology, Baltijskaya St. 8, Moscow 125315, Russia.
  • Burdennyy A; Institute of General Pathology and Pathophysiology, Baltijskaya St. 8, Moscow 125315, Russia.
  • Apanovich P; Research Centre for Medical Genetics, 1 Moskvorechye St., Moscow 115522, Russia.
  • Pronina I; Institute of General Pathology and Pathophysiology, Baltijskaya St. 8, Moscow 125315, Russia.
  • Filippova E; Institute of General Pathology and Pathophysiology, Baltijskaya St. 8, Moscow 125315, Russia.
  • Kazubskaya T; Federal State Budgetary Institution (N.N. Blokhin National Medical Research Center of Oncology) of the Ministry of Health of the Russian Federation, 24 Kashirskoe Shosse, Moscow 115478, Russia.
  • Loginov V; Institute of General Pathology and Pathophysiology, Baltijskaya St. 8, Moscow 125315, Russia.
  • Braga E; Research Centre for Medical Genetics, 1 Moskvorechye St., Moscow 115522, Russia.
  • Alimov A; Institute of General Pathology and Pathophysiology, Baltijskaya St. 8, Moscow 125315, Russia.
Diagnostics (Basel) ; 13(13)2023 Jul 06.
Article en En | MEDLINE | ID: mdl-37443682
Clear cell renal cell carcinoma (ccRCC) is the most common and aggressive histological type of cancer in this location. Distant metastases are present in approximately 30% of patients at the time of first examination. Therefore, the ability to predict the occurrence of metastases in patients at early stages of the disease is an urgent task aimed at personalized treatment. Samples of tumor and paired histologically normal kidney tissue from patients with metastatic and non-metastatic ccRCC were studied. Gene expression was analyzed using real-time PCR. The level of gene methylation was evaluated using bisulfite conversion followed by quantitative methylation-specific PCR. Two groups of genes were analyzed in this study. The first group includes genes whose expression is significantly reduced during metastasis: CA9, NDUFA4L2, EGLN3, and BHLHE41 (p < 0.001, ROC analysis). The second group includes microRNA genes: MIR125B-1, MIR137, MIR375, MIR193A, and MIR34B/C, whose increased methylation levels are associated with the development of distant metastases (p = 0.002 to <0.001, ROC analysis). Based on the data obtained, a combined panel of genes was formed to identify patients whose tumors have a high metastatic potential. The panel can estimate the probability of metastasis with an accuracy of up to 92%.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies / Risk_factors_studies Idioma: En Revista: Diagnostics (Basel) Año: 2023 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies / Risk_factors_studies Idioma: En Revista: Diagnostics (Basel) Año: 2023 Tipo del documento: Article País de afiliación: Rusia Pais de publicación: Suiza