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Characterisation of two snake toxin-targeting human monoclonal immunoglobulin G antibodies expressed in tobacco plants.
Moore, Catherine M; Ljungars, Anne; Paul, Matthew J; Dahl, Camilla Holst; Ahmadi, Shirin; Adams, Anna Christina; Grav, Lise Marie; Schoffelen, Sanne; Voldborg, Bjørn Gunnar; Laustsen, Andreas Hougaard; Ma, Julian K-C.
Afiliación
  • Moore CM; School of Life Sciences, College of Liberal Arts and Sciences, University of Westminster, London, W1W 6UW, United Kingdom. Electronic address: c.moore@westminster.ac.uk.
  • Ljungars A; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark.
  • Paul MJ; Hotung Molecular Immunology Unit, Institute for Infection & Immunity, St George's University of London, Cranmer Terrace, London, SW17 0RE, United Kingdom.
  • Dahl CH; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark.
  • Ahmadi S; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark.
  • Adams AC; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark.
  • Grav LM; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark.
  • Schoffelen S; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark.
  • Voldborg BG; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark.
  • Laustsen AH; Department of Biotechnology and Biomedicine, Technical University of Denmark, Kongens Lyngby, Denmark. Electronic address: ahola@bio.dtu.dk.
  • Ma JK; Hotung Molecular Immunology Unit, Institute for Infection & Immunity, St George's University of London, Cranmer Terrace, London, SW17 0RE, United Kingdom.
Toxicon ; 232: 107225, 2023 Aug 15.
Article en En | MEDLINE | ID: mdl-37442299
Current snakebite antivenoms are based on polyclonal animal-derived antibodies, which can neutralize snake venom toxins in envenomed victims, but which are also associated with adverse reactions. Therefore, several efforts within antivenom research aim to explore the utility of recombinant monoclonal antibodies, such as human immunoglobulin G (IgG) antibodies, which are routinely used in the clinic for other indications. In this study, the feasibility of using tobacco plants as bioreactors for expressing full-length human monoclonal IgG antibodies against snake toxins was investigated. We show that the plant-produced antibodies perform similarly to their mammalian cell-expressed equivalents in terms of in vitro antigen binding. Complete neutralization was achieved by both the plant and mammalian cell-produced anti-α-cobratoxin antibody. The feasibility of using plant-based expression systems may potentially make it easier for laboratories in resource-poor settings to work with human monoclonal IgG antibodies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Mordeduras de Serpientes / Nicotiana Límite: Animals / Humans Idioma: En Revista: Toxicon Año: 2023 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Mordeduras de Serpientes / Nicotiana Límite: Animals / Humans Idioma: En Revista: Toxicon Año: 2023 Tipo del documento: Article Pais de publicación: Reino Unido