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PCR primer design for mitochondrial cox-1 gene from Clinostomum complanatum towards diagnosis.
Misra, Monica; Chauhan, Bhumika; Deepika, Km; Madhuri, A; Sharma, Bindu.
Afiliación
  • Misra M; Department of Zoology, Acharya Narendra Dev College, University of Delhi, India.
  • Chauhan B; Department of Zoology, Acharya Narendra Dev College, University of Delhi, India.
  • Deepika K; Laboratory of Molecular Parasitology, Department of Zoology, Chaudhary Charan Singh University, Meerut, India.
  • Madhuri A; Government Degree College for Women, Begumpet, Hyderabad, India.
  • Sharma B; Laboratory of Molecular Parasitology, Department of Zoology, Chaudhary Charan Singh University, Meerut, India.
Bioinformation ; 18(9): 831-833, 2022.
Article en En | MEDLINE | ID: mdl-37426497
Metacercariae of Clinostomum Leidy, 1856 are frequently encountered in freshwater fish. Clinostomum complanatum is a digenetic zoonotic parasite harbouring the intestine and body cavity of the fishes. 19 human incidences of Clinostomum complanatum infection have been reported to cause pharyngitis and lacramalitis from Japan, Thailand and Korea. Hence, adequate yet effective diagnosis is an issue. Designing primers used in the amplification of genes with adequate specificity and efficiency is of help in diagnosis. Hence, we describe primer design for cox-1 gene from the helminth parasite, Clinostomum complanatum parasitizing the intestine of fish Channastriata (Snakehead murrel). Thus, these designed primers set will be of further use in the wet lab for amplification of concerned gene or DNA fragment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: Bioinformation Año: 2022 Tipo del documento: Article País de afiliación: India Pais de publicación: Singapur
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies Idioma: En Revista: Bioinformation Año: 2022 Tipo del documento: Article País de afiliación: India Pais de publicación: Singapur