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Engineered nickel bioaccumulation in Escherichia coli by NikABCDE transporter and metallothionein overexpression.
Diep, Patrick; Leo Shen, Heping; Wiesner, Julian A; Mykytczuk, Nadia; Papangelakis, Vladimiros; Yakunin, Alexander F; Mahadevan, Radhakrishnan.
Afiliación
  • Diep P; BioZone - Centre for Applied Bioscience and Bioengineering, Department of Chemical Engineering and Applied Chemistry University of Toronto Toronto Canada.
  • Leo Shen H; BioZone - Centre for Applied Bioscience and Bioengineering, Department of Chemical Engineering and Applied Chemistry University of Toronto Toronto Canada.
  • Wiesner JA; MIRARCO Mining Innovation Laurentian University Sudbury Canada.
  • Mykytczuk N; MIRARCO Mining Innovation Laurentian University Sudbury Canada.
  • Papangelakis V; BioZone - Centre for Applied Bioscience and Bioengineering, Department of Chemical Engineering and Applied Chemistry University of Toronto Toronto Canada.
  • Yakunin AF; BioZone - Centre for Applied Bioscience and Bioengineering, Department of Chemical Engineering and Applied Chemistry University of Toronto Toronto Canada.
  • Mahadevan R; Centre for Environmental Biotechnology School of Natural Sciences University of Bangor Wales UK.
Eng Life Sci ; 23(7): 2200133, 2023 Jul.
Article en En | MEDLINE | ID: mdl-37408871
Mine wastewater often contains dissolved metals at concentrations too low to be economically extracted by existing technologies, yet too high for environmental discharge. The most common treatment is chemical precipitation of the dissolved metals using limestone and subsequent disposal of the sludge in tailing impoundments. While it is a cost-effective solution to meet regulatory standards, it represents a lost opportunity. In this study, we engineered Escherichia coli to overexpress its native NikABCDE transporter and a heterologous metallothionein to capture nickel at concentrations in local effluent streams. We found the engineered strain had a 7-fold improvement in the bioaccumulation performance for nickel compared to controls, but also observed a drastic decrease in cell viability due to metabolic burden or inducer (IPTG) toxicity. Growth kinetic analysis revealed the IPTG concentrations used based on past studies lead to growth inhibition, thus delineating future avenues for optimization of the engineered strain and its growth conditions to perform in more complex environments.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Guideline Idioma: En Revista: Eng Life Sci Año: 2023 Tipo del documento: Article Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Guideline Idioma: En Revista: Eng Life Sci Año: 2023 Tipo del documento: Article Pais de publicación: Alemania