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Rapid detection of fluoroquinolone resistance in Mycobacterium tuberculosis using a novel multienzyme isothermal rapid assay.
Li, Ma-Chao; Lu, Yao; Liu, Hai-Can; Lin, Shi-Qiang; Qian, Cheng; Nan, Xiao-Tian; Li, Gui-Lian; Zhao, Xiu-Qin; Wan, Kang-Lin; Zhao, Li-Li.
Afiliación
  • Li MC; State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China.
  • Lu Y; Department of Laboratory Medicine, Ningbo First Hospital, Ningbo Hospital of Ningbo University, Ningbo, 315010, China.
  • Liu HC; State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China.
  • Lin SQ; College of Life Science, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
  • Qian C; Beijing Center for Disease Control and Prevention, 100013, Beijing, China.
  • Nan XT; State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China.
  • Li GL; State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China.
  • Zhao XQ; State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China.
  • Wan KL; State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China.
  • Zhao LL; State Key Laboratory of Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, 102206, Beijing, China. zhaolili@icdc.cn.
J Antibiot (Tokyo) ; 76(10): 598-602, 2023 10.
Article en En | MEDLINE | ID: mdl-37402884
Simple, rapid, and accurate detection of Fluoroquinolone (FQ) resistance is essential for early initiation of appropriate anti-tuberculosis treatment regimen among rifampicin-resistant tuberculosis (RR-TB). In this study, we developed a new assay, which combines multienzyme isothermal rapid amplification and a lateral flow strip (MIRA-LF), to identify the mutations on codons 90 and 94 of gyrA for detecting levofloxacin (LFX) resistance. Compared to conventional phenotypic drug susceptibility testing, the new assay detected fluoroquinolone resistance with a sensitivity, specificity, and accuracy of 92.4%, 98.5%, and 96.5%, respectively. Thus, these characteristics of the newly developed MIRA-LF assay make it particularly useful and accurate for detecting FQ resistance in Mycobacterium tuberculosis in resource-limited condition.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tuberculosis Resistente a Múltiples Medicamentos / Mycobacterium tuberculosis Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: J Antibiot (Tokyo) Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Tuberculosis Resistente a Múltiples Medicamentos / Mycobacterium tuberculosis Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Humans Idioma: En Revista: J Antibiot (Tokyo) Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido