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Molecular investigation of bovine tropical theileriosis outbreak in an organized dairy cattle farm in Madhya Pradesh, India.
Verma, Rupesh; Das, Giridhari; Kumar, Suman; Nath, Subhradal; Rai, Ajay; Soni, Ashish; Mandal, Sanju.
Afiliación
  • Verma R; Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, South Civil Lines, Jabalpur, MP, 482001, India. vrupesh77@gmail.com.
  • Das G; Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, South Civil Lines, Jabalpur, MP, 482001, India.
  • Kumar S; Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, South Civil Lines, Jabalpur, MP, 482001, India.
  • Nath S; Department of Veterinary Parasitology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, South Civil Lines, Jabalpur, MP, 482001, India.
  • Rai A; Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, South Civil Lines, Jabalpur, MP, 482001, India.
  • Soni A; Department of Veterinary Medicine, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, South Civil Lines, Jabalpur, MP, 482001, India.
  • Mandal S; Department of Veterinary Biochemistry, College of Veterinary Science and Animal Husbandry, Nanaji Deshmukh Veterinary Science University, South Civil Lines, Jabalpur, MP, 482001, India.
Parasitol Res ; 122(9): 2079-2089, 2023 Sep.
Article en En | MEDLINE | ID: mdl-37401931
The present study aimed to investigate an outbreak of Theileria annulata (T. annulata) infection in an organized dairy cattle farm in Madhya Pradesh, India, using clinical and molecular techniques. Following the deaths of two crossbred cattle in March 2021, 43 blood samples were collected from infected and apparently healthy animals and examined by blood smear and polymerase chain reaction (PCR) techniques. The blood smear examination showed that 23.25% of samples were positive for Theileria organisms, while conventional PCR targeting the 18S ribosomal RNA (18S rRNA) and T. annulata merozoite surface antigen-1 (TAMS-1) genes revealed that 32.55% of samples were positive for T. annulata. PCR targeting cytochrome b (Cytb) gene showed 46.51% of samples were positive for T. annulata. Haematological analysis confirmed clinical signs of infection in affected animals, which were treated with buparvaquone @ 2.5 mg/kg body weight intramuscularly along with supportive medicine. Two 18S rRNA gene amplicons were sequenced and analysed in a phylogenetic tree and haplotype network with 54 Indian and 38 foreign sequences. The phylogenetic tree revealed two groups with a high posterior probability and bootstrap value, while the haplotype network revealed 35 haplotypes, with haplotype 1 (H1) being the most abundant and several single haplotypes clustering around it, indicating fast and widespread expansion. Genetic diversity indices and neutrality tests confirmed that the population was expanding. These studies highlight the significance of prompt and precise diagnosis and management of T. annulata outbreaks and provide insights into its evolutionary history and population dynamics of T. annulata in India, which could aid improving disease preventive and control strategies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Theileriosis / Enfermedades de los Bovinos / Theileria annulata Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Parasitol Res Asunto de la revista: PARASITOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: India Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Theileriosis / Enfermedades de los Bovinos / Theileria annulata Límite: Animals País/Región como asunto: Asia Idioma: En Revista: Parasitol Res Asunto de la revista: PARASITOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: India Pais de publicación: Alemania