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An Intranasal Multivalent Epitope-Based Nanoparticle Vaccine Confers Broad Protection against Divergent Influenza Viruses.
Pan, Jingdi; Wang, Qihui; Qi, Mi; Chen, Jianjun; Wu, Xuefan; Zhang, Xiaowei; Li, Wei; Zhang, Xian-En; Cui, Zongqiang.
Afiliación
  • Pan J; State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, People's Republic of China.
  • Wang Q; University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
  • Qi M; State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, People's Republic of China.
  • Chen J; University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
  • Wu X; State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, People's Republic of China.
  • Zhang X; University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
  • Li W; State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, People's Republic of China.
  • Zhang XE; University of Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
  • Cui Z; State Key Laboratory of Virology, Wuhan Institute of Virology, Center for Biosafety Mega-Science, Chinese Academy of Sciences, Wuhan 430071, People's Republic of China.
ACS Nano ; 17(14): 13474-13487, 2023 07 25.
Article en En | MEDLINE | ID: mdl-37395606
The development of a universal influenza vaccine to control public health threats from circulating and emerging influenza viruses is highly desirable. Here we report an intranasal multivalent epitope-based nanoparticle vaccine with broad protection against divergent influenza A and B viruses. Three highly conserved epitopes consisting of the A α-helix of hemagglutinin (H), the ectodomain of matrix protein 2 (M) and the HCA-2 of neuraminidase (N) are presented on a self-assembling recombinant human heavy chain ferritin cage (F) to generate the HMNF nanoparticle. Intranasal immunization of mice with HMNF mobilized potent immune responses, including high levels of antigen-specific antibodies and T cell-mediated responses, which exhibited cross-reactivity to various antigen mutations. Vaccination with HMNF conferred full protection against lethal challenge with divergent influenza A and B viruses. The broad protection of HMNF nanoparticles could be attributed to the synergistic function of antibodies and T cells. Moreover, the induced immune responses are long-lasting, and protection is maintained six months after vaccination. Our constructed HMNF nanoparticle can serve as a promising candidate for a universal influenza vaccine.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Orthomyxoviridae / Vacunas contra la Influenza / Infecciones por Orthomyxoviridae / Gripe Humana / Nanopartículas Límite: Animals / Humans Idioma: En Revista: ACS Nano Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Orthomyxoviridae / Vacunas contra la Influenza / Infecciones por Orthomyxoviridae / Gripe Humana / Nanopartículas Límite: Animals / Humans Idioma: En Revista: ACS Nano Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos