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Flow behavior of protein solutions in a lab-scale chromatographic system.
Filip, Bartlomiej; Bochenek, Roman; Marek, Wojciech K; Antos, Dorota.
Afiliación
  • Filip B; Doctoral School of the Rzeszów University of Technology, Rzeszów, Poland.
  • Bochenek R; Department of Chemical and Process Engineering, Rzeszów University of Technology, Rzeszów, Poland.
  • Marek WK; Department of Chemical and Process Engineering, Rzeszów University of Technology, Rzeszów, Poland.
  • Antos D; Department of Chemical and Process Engineering, Rzeszów University of Technology, Rzeszów, Poland. Electronic address: dorota.antos@prz.edu.pl.
J Chromatogr A ; 1705: 464178, 2023 Aug 30.
Article en En | MEDLINE | ID: mdl-37392636
A fluid dynamics model has been developed to describe flow behavior in a lab-scale chromatographic system dedicated for protein processing. The case study included a detailed analysis of elution pattern of a protein, which was a monoclonal antibody, glycerol, and their mixtures in aqueous solutions. Glycerol solutions mimicked viscous environment of the concentrated protein solutions. The model accounted for concentration dependences of solution viscosity and density, and dispersion anisotropy in the packed bed. It was implemented into a commercial computational fluid dynamics software using user-defined functions. The prediction efficiency was successfully verified by comparing the model simulations in the form of the concentration profiles and their variances with the corresponding experimental data. The contribution of the individual elements of the chromatographic system to protein band broadening was evaluated for different configurations: for the extra-column volumes in the absence of the chromatographic column, for the zero-length column without the packed bed and for the column containing the packed bed. The influence of the operating variables, including: the mobile phase flowrate, the type of the injection system, i.e., the injection loop capillary or the superloop, the injection volume and the length of the packed bed, on band broadening of the protein was determined under nonadsorbing conditions. For protein solutions having viscosity comparable with the mobile phase, the flow behavior either in the column hardware or in the injection system made major contributions to band broadening, which depended on the type of the injection system. For highly viscous protein solution, the flow behavior in the packed bed exerted a dominant influence on band broadening.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromatografía / Glicerol Tipo de estudio: Prognostic_studies Idioma: En Revista: J Chromatogr A Año: 2023 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cromatografía / Glicerol Tipo de estudio: Prognostic_studies Idioma: En Revista: J Chromatogr A Año: 2023 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Países Bajos