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Molecular classification of muscle-invasive bladder cancer based on a simplified immunohistochemical panel using GATA3, CK5/6 and p16.
Terlevic, Robert; Ulamec, Monika; Stimac, Goran; Murgic, Jure; Kruslin, Bozo.
Afiliación
  • Terlevic R; Department of Pathology and Cytology, Pula General Hospital, Pula, Croatia.
  • Ulamec M; Clinical Department of Pathology and Cytology "Ljudevit Jurak", Clinical Hospital Center Sestre milosrdnice, Zagreb, Croatia; School of Medicine, University of Zagreb, Zagreb, Croatia.
  • Stimac G; Clinical Department of Urology, Clinical Hospital Center Sestre milosrdnice, Zagreb, Croatia.
  • Murgic J; School of Medicine, University of Zagreb, Zagreb, Croatia; Clinical Department of Oncology, Clinical Hospital Center Sestre milosrdnice, Zagreb, Croatia.
  • Kruslin B; Clinical Department of Pathology and Cytology "Ljudevit Jurak", Clinical Hospital Center Sestre milosrdnice, Zagreb, Croatia; School of Medicine, University of Zagreb, Zagreb, Croatia.
Biomol Biomed ; 23(6): 968-975, 2023 Nov 03.
Article en En | MEDLINE | ID: mdl-37389960
The choice of therapy for muscle-invasive bladder cancer (MIBC) could be influenced by the tumor's molecular subtype. Currently, well-defined consensus subtypes are based on tumor microarray mRNA data. Clearly defined and easy-to-use surrogate molecular subtypes, based on immunohistochemistry (IHC) performed on whole slides, are needed to make subtyping cost-effective and useful in routine work and future research. To aid in the development of a simple immunohistochemical classifier, a retrospective single-center series of 92 cases of localized bladder cancer was identified. Routine IHC for GATA3, cytokeratins 5 and 6 (CK5/6), and p16 was performed on whole tissue blocks containing muscle-invasive disease. Electronic medical records were retrieved and searched for clinical variables, treatment, and survival data. The mean age was 69.6 years, and 73% were males. Conservative treatment was used in 55% of cases, while cystectomy with chemotherapy was used in 45%. GATA3 and CK5/6 expression divided cases into broad luminal and basal subtypes, respectively, while p16 expression was used to subclassify luminal cases into luminal papillary and luminal unstable types according to the consensus molecular classification. When subtyped in this way, GATA3 and CK5/6 negative cases showed worse overall survival. Molecular subtyping of MIBC on whole slides containing muscle-invasive tumor using only three commonly used, consensus-based antibodies, is a feasible and cost-effective method for detecting subtypes of invasive bladder cancer. Future work combining morphological analysis and IHC is needed to fully translate the consensus molecular classification into a comprehensive, cost-effective subtyping strategy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Vejiga Urinaria / Neoplasias de los Músculos Tipo de estudio: Prognostic_studies Límite: Aged / Female / Humans / Male Idioma: En Revista: Biomol Biomed Año: 2023 Tipo del documento: Article País de afiliación: Croacia Pais de publicación: Bosnia-Herzegovina
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias de la Vejiga Urinaria / Neoplasias de los Músculos Tipo de estudio: Prognostic_studies Límite: Aged / Female / Humans / Male Idioma: En Revista: Biomol Biomed Año: 2023 Tipo del documento: Article País de afiliación: Croacia Pais de publicación: Bosnia-Herzegovina