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RNase H1 facilitates recombinase recruitment by degrading DNA-RNA hybrids during meiosis.
Liu, Chao; Wang, Liying; Li, Yanan; Guo, Mengmeng; Hu, Jun; Wang, Teng; Li, Mengjing; Yang, Zhuo; Lin, Ruoyao; Xu, Wei; Chen, Yinghong; Luo, Mengcheng; Gao, Fei; Chen, Jia-Yu; Sun, Qianwen; Liu, Hongbin; Sun, Bo; Li, Wei.
Afiliación
  • Liu C; Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, China.
  • Wang L; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Stem Cell and Regenerative Medicine Innovation Institute, Chinese Academy of Sciences, Beijing 100101, China.
  • Li Y; Guangzhou Women and Children's Medical Center, Guangzhou Medical University, Guangzhou 510623, China.
  • Guo M; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
  • Hu J; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Stem Cell and Regenerative Medicine Innovation Institute, Chinese Academy of Sciences, Beijing 100101, China.
  • Wang T; University of Chinese Academy of Sciences, Beijing 100049, China.
  • Li M; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China.
  • Yang Z; School of Life Science and Technology, ShanghaiTech University, Shanghai 201210, China.
  • Lin R; Center for Reproductive Medicine, Shandong University, Jinan 250012, China.
  • Xu W; Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
  • Chen Y; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China.
  • Luo M; Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing 100084, China.
  • Gao F; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Stem Cell and Regenerative Medicine Innovation Institute, Chinese Academy of Sciences, Beijing 100101, China.
  • Chen JY; University of Chinese Academy of Sciences, Beijing 100049, China.
  • Sun Q; Department of Tissue and Embryology, School of Basic Medical Sciences, Wuhan University, Hubei Provincial Key Laboratory of Developmentally Originated Disease, Wuhan 430072, China.
  • Liu H; State Key Laboratory of Stem Cell and Reproductive Biology, Institute of Zoology, Stem Cell and Regenerative Medicine Innovation Institute, Chinese Academy of Sciences, Beijing 100101, China.
  • Sun B; University of Chinese Academy of Sciences, Beijing 100049, China.
  • Li W; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Chemistry and Biomedicine Innovation Center (ChemBIC), Nanjing University, Nanjing 210023, China.
Nucleic Acids Res ; 51(14): 7357-7375, 2023 08 11.
Article en En | MEDLINE | ID: mdl-37378420
DNA-RNA hybrids play various roles in many physiological progresses, but how this chromatin structure is dynamically regulated during spermatogenesis remains largely unknown. Here, we show that germ cell-specific knockout of Rnaseh1, a specialized enzyme that degrades the RNA within DNA-RNA hybrids, impairs spermatogenesis and causes male infertility. Notably, Rnaseh1 knockout results in incomplete DNA repair and meiotic prophase I arrest. These defects arise from the altered RAD51 and DMC1 recruitment in zygotene spermatocytes. Furthermore, single-molecule experiments show that RNase H1 promotes recombinase recruitment to DNA by degrading RNA within DNA-RNA hybrids and allows nucleoprotein filaments formation. Overall, we uncover a function of RNase H1 in meiotic recombination, during which it processes DNA-RNA hybrids and facilitates recombinase recruitment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ribonucleasa H / Meiosis Límite: Humans / Male Idioma: En Revista: Nucleic Acids Res Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ribonucleasa H / Meiosis Límite: Humans / Male Idioma: En Revista: Nucleic Acids Res Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido