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An Analysis of DNA Sequence Polymorphism in the Swamp Buffalo Toll-like Receptor (TLR2) Gene.
Kenchaiwong, Wootichai; Pongthaisong, Pongphol; Kananit, Srinuan; Duangjinda, Monchai; Boonkum, Wuttigrai.
Afiliación
  • Kenchaiwong W; Network Center for Animal Breeding and Omics Research, Khon Kaen University, Khon Kaen 40002, Thailand.
  • Pongthaisong P; Small Ruminant Research Unit, Faculty of Veterinary Science, Mahasarakham University, Mahasarakham 44000, Thailand.
  • Kananit S; Small Ruminant Research Unit, Faculty of Veterinary Science, Mahasarakham University, Mahasarakham 44000, Thailand.
  • Duangjinda M; Network Center for Animal Breeding and Omics Research, Khon Kaen University, Khon Kaen 40002, Thailand.
  • Boonkum W; Department of Animal Science, Faculty of Agriculture, Khon Kean University, Khon Kean 40002, Thailand.
Animals (Basel) ; 13(12)2023 Jun 16.
Article en En | MEDLINE | ID: mdl-37370522
Toll-like receptors (TLRs) are transmembrane proteins important for directing immune responses. Their primary role is to recognize pathogens based on single-nucleotide polymorphism (SNP) characteristics. TLR2 is categorized as a pattern recognition receptor (PRR) that is important for the recognition of pathogens. Nucleotide variation in the coding region determines the conformation of the TLR protein, affecting its protein domain efficiency. This study aimed to identify SNPs in the coding region of TLR2 to enhance available genetic tools for improving health and production in swamp buffalo. A total of 50 buffaloes were randomly sampled from the northeastern part of Thailand for genomic DNA extraction and sequencing. Nucleotide sequences were aligned and compared with cattle and river buffalo based on the database. The results showed, there were 29 SNP locations in swamp buffalo and 14 different locations in both cattle and buffaloes. Haplotype analysis revealed that 27 haplotypes occurred. Swamp buffalo were identified from 13 SNPs based on biallelic analysis, which found eight synonymous and five nonsynonymous SNPs. Nucleotide diversity (π) was 0.16, indicating genetic diversity. Genetic diversity (haplotype diversity; HD) was high at 0.99 ± 0.04. This indicates a high probability that the two sample haplotypes are different. The π and HD values are important indicators of the genetic diversity of the swamp buffalo population. In summary, the Thai swamp buffalo population detected a polymorphism of the coding region of the TRL2 gene. Therefore, further, in-depth study of the relationship between these genes in the immune system and disease resistance should be recommended.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Animals (Basel) Año: 2023 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Animals (Basel) Año: 2023 Tipo del documento: Article País de afiliación: Tailandia Pais de publicación: Suiza