A simplified procedure for the purification of large quantities of fetal bovine serum acetylcholinesterase.
Life Sci
; 39(3): 195-9, 1986 Jul 21.
Article
en En
| MEDLINE
| ID: mdl-3736320
A simple procedure has been developed for the large scale purification of fetal bovine serum acetylcholinesterase (AChE) (EC 3.1.1.7). The procedure involves two steps: batch adsorption of the AChE from 250 L of serum onto a procainamide affinity Sepharose 4B gel; and analytical procainamide affinity chromatography of the step-1 product. Over 100 mg of AChE was purified in 10 days to apparent homogeneity with this procedure.
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Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Acetilcolinesterasa
Límite:
Animals
Idioma:
En
Revista:
Life Sci
Año:
1986
Tipo del documento:
Article
Pais de publicación:
Países Bajos