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Roles of human periodontal ligament stem cells in osteogenesis and inflammation in periodontitis models: Effect of 1α,25-dihydroxyvitamin D3.
Wang, Jing-Jiao; Zhang, Cheng-Lei; Guo, Xiao-Qian; Yang, Chang-Yi.
Afiliación
  • Wang JJ; Department of Periodontics, Stomatological Hospital, General Hospital of Ningxia Medical University, Yinchuan 750003, Ningxia, China.
  • Zhang CL; Medical Laboratory, General Hospital of Ningxia Medical University, Yinchuan 750003, Ningxia, China. Electronic address: aries140aa@163.com.
  • Guo XQ; Department of Periodontics, Stomatological Hospital, General Hospital of Ningxia Medical University, Yinchuan 750003, Ningxia, China.
  • Yang CY; Department of Periodontics, Stomatological Hospital, General Hospital of Ningxia Medical University, Yinchuan 750003, Ningxia, China.
J Steroid Biochem Mol Biol ; 232: 106347, 2023 09.
Article en En | MEDLINE | ID: mdl-37331433
Periodontitis is a chronic inflammatory disease caused by Porphyromonas gingivalis and other bacteria, and human periodontal ligament stem cells (hPDLSCs) are a promising candidate for the treatment of periodontal supporting tissue defects. This study aimed to investigate the effect of 1α,25-dihydroxyvitamin D3 [1,25(OH)2VitD3] on osteogenic differentiation of hPDLSCs in an in vitro periodontitis model and whether it can improve inflammatory status. hPDLSCs were in vitro isolated and identified. After treatment with 1,25(OH)2VitD3 and ultrapure pure Porphyromonas gingivalis lipopolysaccharide (LPS-G), the viability of hPDLSCs was detected using Cell Counting Kit-8, the expressions of osteogenic markers and inflammatory genes using Western blotting and quantitative reverse transcription PCR (qRT-PCR), the levels of inflammatory factors in cells using enzyme linked immunosorbent assay (ELISA), and the fluorescence signal intensity of osteoblastic markers and inflammatory genes in cells using immunofluorescence assay. It was found that 1,25(OH)2VitD3 reversed the inhibition of hPDLSCs proliferation by LPS-G; LPS-G exhibited inhibitory effect on ALP, Runx2, and OPN expressions, and such inhibitory effect was significantly weakened when co-acting with 1,25(OH)2VitD3. Meanwhile, LPS-G upregulated the expressions of inflammatory genes IL-1ß and Casp1, whereas 1,25(OH)2VitD3 antagonized such an effect and improved the inflammatory status. In conclusion, 1,25(OH)2VitD3 can reverse the inhibitory effect of LPS-G on hPDLSCs proliferation and osteogenic differentiation and suppress LPS-G-induced upregulation of inflammatory gene expressions.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Periodontitis Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: J Steroid Biochem Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR / BIOQUIMICA Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteogénesis / Periodontitis Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: J Steroid Biochem Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR / BIOQUIMICA Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido