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Mapping of a soybean rust resistance in PI 594756 at the Rpp1 locus.
Barros, Luciane G; Avelino, Bruna B; da Silva, Danielle C G; Ferreira, Everton G C; Castanho, Fernanda M; Ferreira, Marcio E; Lopes-Caitar, Valeria S; Marin, Silvana R R; Arias, Carlos A A; de O N Lopes, Ivani; Abdelnoor, Ricardo V; Marcelino-Guimarães, Francismar C.
Afiliación
  • Barros LG; Department of General Biology, State University of Londrina (UEL), Londrina, Paraná Brazil.
  • Avelino BB; Department of General Biology, State University of Londrina (UEL), Londrina, Paraná Brazil.
  • da Silva DCG; Brazilian Agricultural Research Corporation (Embrapa), Embrapa Soybean, Londrina, Paraná Brazil.
  • Ferreira EGC; Department of General Biology, State University of Londrina (UEL), Londrina, Paraná Brazil.
  • Castanho FM; Department of General Biology, State University of Londrina (UEL), Londrina, Paraná Brazil.
  • Ferreira ME; Brazilian Agricultural Research Coorporation, Embrapa Genetic Resources and Biotechnology, Brasilia, Distrito Federal Brazil.
  • Lopes-Caitar VS; Brazilian Agricultural Research Corporation (Embrapa), Embrapa Soybean, Londrina, Paraná Brazil.
  • Marin SRR; Brazilian Agricultural Research Corporation (Embrapa), Embrapa Soybean, Londrina, Paraná Brazil.
  • Arias CAA; Brazilian Agricultural Research Corporation (Embrapa), Embrapa Soybean, Londrina, Paraná Brazil.
  • de O N Lopes I; Brazilian Agricultural Research Corporation (Embrapa), Embrapa Soybean, Londrina, Paraná Brazil.
  • Abdelnoor RV; Brazilian Agricultural Research Corporation (Embrapa), Embrapa Soybean, Londrina, Paraná Brazil.
  • Marcelino-Guimarães FC; Brazilian Agricultural Research Corporation (Embrapa), Embrapa Soybean, Londrina, Paraná Brazil.
Mol Breed ; 43(2): 12, 2023 Feb.
Article en En | MEDLINE | ID: mdl-37313128
Asian soybean rust (ASR), caused by the fungus Phakopsora pachyrhizi, is the main disease affecting soybean in Brazil. This study aimed at investigating and mapping the resistance of the PI 594756 to P. pachyrhizi, by using Bulked Segregant Analysis (BSA). The PI 594756 and the susceptible PI 594891 were crossed and the resulting F2 and F2:3 populations (208 and 1770 plants, respectively) were tested against ASR. Also, these PIs and differential varieties were tested against a panel of monosporic isolates. Plants presenting tan lesions were classified as susceptible (S) while plants presenting reddish-brown (RB) lesions were classified as resistant. DNA bulks were genotyped with Infinium BeadChips and the genomic region identified was further analyzed in the F2 individuals with target GBS (tGBS). PI 594,56 presented a unique resistance profile compared to the differential varieties. The resistance was monogenic dominant; however, it was classified as incompletely dominant when quantitatively studied. Genetic and QTL mapping placed the PI 594756 gene between the genomic region located at 55,863,741 and 56,123,516 bp of chromosome 18. This position is slightly upstream mapping positions of Rpp1 (PI 200492) and Rpp1-b (PI 594538A). Finally, we performed a haplotype analysis in a whole genomic sequencing-SNP database composed of Brazilian historical germplasm and sources of Rpp genes. We found SNPs that successfully differentiated the new PI 594756 allele from Rpp1 and Rpp1-b sources. The haplotype identified can be used as a tool for marker-assisted selection (MAS). Supplementary Information: The online version contains supplementary material available at 10.1007/s11032-023-01358-4.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Mol Breed Año: 2023 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Mol Breed Año: 2023 Tipo del documento: Article Pais de publicación: Países Bajos