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SARS-CoV2 infection induce miR-155 expression and skewed Th17/Treg balance by changing SOCS1 level: A clinical study.
Soltani-Zangbar, Mohammad Sadegh; Hajivalili, Mahsa; Daneshdoust, Danyal; Ghadir, Sara; Savari, Golaleh; Zolfaghari, Mohammadali; Aghebati-Maleki, Leili; Oloufi, Solmaz; Nouri, Narjes; Amini, Naser; Mehdizadeh, Amir; Ghasemi Moghadam, Hossein; Mahmoodpoor, Ata; Ahmadian Heris, Javad; Yousefi, Mehdi.
Afiliación
  • Soltani-Zangbar MS; Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Hajivalili M; Behbahan Faculty of Medical Sciences, Behbahan, Iran.
  • Daneshdoust D; Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
  • Ghadir S; Student Research Committee, Faculty of Medical Sciences, Babol University of Medical Sciences, Babol, Iran.
  • Savari G; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Zolfaghari M; Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Aghebati-Maleki L; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Oloufi S; School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran; School of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
  • Nouri N; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Amini N; Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran; Institute of Regenerative Medicine, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
  • Mehdizadeh A; Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Ghasemi Moghadam H; Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Mahmoodpoor A; Department of Anesthesiology, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Ahmadian Heris J; Department of Allergy and Clinical Immunology, Pediatric Hospital, Tabriz University of Medical Sciences, Tabriz, Iran.
  • Yousefi M; Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Immunology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran. Electronic address: Yousefime@tbzmed.ac.ir.
Cytokine ; 169: 156248, 2023 09.
Article en En | MEDLINE | ID: mdl-37307689
BACKGROUND: One of the regulators in severe acute respiratory syndrome coronavirus2 (SARS-CoV2) infection is miRNAs. In COVID-19 patients, immunological responses to SARS-CoV2 infection may be impacted by miR-155, a miRNA associated to inflammation. MATERIALS AND METHODS: Peripheral blood mononuclear cells (PBMCs) of 50 confirmed COVID-19 patients /Healthy Controls (HCs) was isolated by Ficoll. The frequency of T helper 17 and regulatory T cells was analyzed by flowcytometry. The RNA was extracted from each sample and after synthesis of c-DNA, the relative expression of miR-155, suppressor of cytokine signaling (SOCS-1), Signal transducer and activator of transcription 3(STAT3), and Fork Head Box Protein 3 (FoxP3) was evaluated by real-time PCR. The protein level of STAT3, FoxP3 and RORγT in the isolated PBMCs measured by western blotting. The serum level of IL-10, TGF-ß, IL-17 and IL21 was assessed by ELISA method. RESULTS: The population of Th17 cells showed a significant rise, whereas Treg cells reduced in COVID-19 cases. The master transcription factor of Treg (FoxP3) and Th17 (RORγT) relative expression showed the same pattern as flowcytometry. STAT3 level of expression at RNA and protein level increased in COVID-19 cases. FOXP3 and SOCS-1 proteins were down-regulated. The relative expression of miR-155, up-regulated in PBMC of COVID-19 patients and revealed a negative correlation with SOCS-1. The serum cytokine profile showed a reduction in TGF-ß, on the other hand an increase was seen in IL-17, IL-21 and IL-10 in COVID-19 cases toward control group. CONCLUSION: Based on the studies conducted in this field, it can be suggested that Th17/Treg in covid-19 patients can be affected by miR-155 and it can be considered a valuable diagnostic and prognostic factor in this disease.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos T Reguladores / MicroARNs / Células Th17 / Proteína 1 Supresora de la Señalización de Citocinas / COVID-19 Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Cytokine Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Linfocitos T Reguladores / MicroARNs / Células Th17 / Proteína 1 Supresora de la Señalización de Citocinas / COVID-19 Tipo de estudio: Prognostic_studies Límite: Humans Idioma: En Revista: Cytokine Asunto de la revista: ALERGIA E IMUNOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Irán Pais de publicación: Reino Unido