Solid-Phase Cell-Free Protein Synthesis and Its Applications in Biotechnology.
Adv Biochem Eng Biotechnol
; 185: 21-46, 2023.
Article
en En
| MEDLINE
| ID: mdl-37306703
Cell-free systems for the in vitro production of proteins have revolutionized the synthetic biology field. In the last decade, this technology is gaining momentum in molecular biology, biotechnology, biomedicine and even education. Materials science has burst into the field of in vitro protein synthesis to empower the value of existing tools and expand its applications. In this sense, the combination of solid materials (normally functionalized with different biomacromolecules) together with cell-free components has made this technology more versatile and robust. In this chapter, we discuss the combination of solid materials with DNA and transcription-translation machinery to synthesize proteins within compartments, to immobilize and purify in situ the nascent protein, to transcribe and transduce DNAs immobilized on solid surfaces, and the combination of all or some of these strategies.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Biotecnología
/
Biología Molecular
Idioma:
En
Revista:
Adv Biochem Eng Biotechnol
Año:
2023
Tipo del documento:
Article
País de afiliación:
España
Pais de publicación:
Alemania