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The human discs large protein 1 interacts with and maintains connexin 43 at the plasma membrane in keratinocytes.
Scott, Harry; Dong, Li; Stevenson, Andrew; MacDonald, Alasdair I; Srinivasan, Sharmila; Massimi, Paola; Banks, Lawrence; Martin, Patricia E; Johnstone, Scott R; Graham, Sheila V.
Afiliación
  • Scott H; MRC-University of Glasgow Centre for Virus Research, School of Infection and Immunity, College of Medical Veterinary and Life Sciences, University of Glasgow, Garscube Estate, Glasgow G61 1QH, UK.
  • Dong L; MRC-University of Glasgow Centre for Virus Research, School of Infection and Immunity, College of Medical Veterinary and Life Sciences, University of Glasgow, Garscube Estate, Glasgow G61 1QH, UK.
  • Stevenson A; MRC-University of Glasgow Centre for Virus Research, School of Infection and Immunity, College of Medical Veterinary and Life Sciences, University of Glasgow, Garscube Estate, Glasgow G61 1QH, UK.
  • MacDonald AI; MRC-University of Glasgow Centre for Virus Research, School of Infection and Immunity, College of Medical Veterinary and Life Sciences, University of Glasgow, Garscube Estate, Glasgow G61 1QH, UK.
  • Srinivasan S; Translation Research Platform for Veterinary Biologicals, Chennai, Tamil Nadu, India.
  • Massimi P; International Centre for Genetic Engineering and Biotechnology, Trieste, Italy.
  • Banks L; International Centre for Genetic Engineering and Biotechnology, Trieste, Italy.
  • Martin PE; Department of Biological and Biomedical Sciences, School of Health and Life Sciences, Glasgow Caledonian University, Glasgow G4 0BA, UK.
  • Johnstone SR; Fralin Biomedical Research Institute at Virginia Tech Carilion, Center for Vascular and Heart Research, Virginia Tech, Roanoke VA 24016, USA.
  • Graham SV; MRC-University of Glasgow Centre for Virus Research, School of Infection and Immunity, College of Medical Veterinary and Life Sciences, University of Glasgow, Garscube Estate, Glasgow G61 1QH, UK.
J Cell Sci ; 136(11)2023 06 01.
Article en En | MEDLINE | ID: mdl-37288673
Gap junction channels, composed of connexins, allow direct cell-to-cell communication. Connexin 43 (Cx43; also known as GJA1) is widely expressed in tissues, including the epidermis. In a previous study of human papillomavirus-positive cervical epithelial tumour cells, we identified Cx43 as a binding partner of the human homologue of Drosophila Discs large (Dlg1; also known as SAP97). Dlg1 is a member of the membrane associated-guanylate kinase (MAGUK) scaffolding protein family, which is known to control cell shape and polarity. Here, we show that Cx43 also interacts with Dlg1 in uninfected keratinocytes in vitro and in keratinocytes, dermal cells and adipocytes in normal human epidermis in vivo. Depletion of Dlg1 in keratinocytes did not alter Cx43 transcription but was associated with a reduction in Cx43 protein levels. Reduced Dlg1 levels in keratinocytes resulted in a reduction in Cx43 at the plasma membrane with a concomitant reduction in gap junctional intercellular communication and relocation of Cx43 to the Golgi compartment. Our data suggest a key role for Dlg1 in maintaining Cx43 at the plasma membrane in keratinocytes.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Queratinocitos / Conexina 43 / Homólogo 1 de la Proteína Discs Large Límite: Humans Idioma: En Revista: J Cell Sci Año: 2023 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Queratinocitos / Conexina 43 / Homólogo 1 de la Proteína Discs Large Límite: Humans Idioma: En Revista: J Cell Sci Año: 2023 Tipo del documento: Article Pais de publicación: Reino Unido