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Generation of LexA enhancer-trap lines in Drosophila by an international scholastic network.
Kim, Ella S; Rajan, Arjun; Chang, Kathleen; Govindarajan, Sanath; Gulick, Clara; English, Eva; Rodriguez, Bianca; Bloomfield, Orion; Nakada, Stella; Beard, Charlotte; O'Connor, Sarah; Mastroianni, Sophia; Downey, Emma; Feigenbaum, Matthew; Tolentino, Caitlin; Pace, Abigail; Khan, Marina; Moon, Soyoun; DiPrima, Jordan; Syed, Amber; Lin, Flora; Abukhadra, Yasmina; Bacon, Isabella; Beckerle, John; Cho, Sophia; Donkor, Nana Esi; Garberg, Lucy; Harrington, Ava; Hoang, Mai; Lawani, Nosa; Noori, Ayush; Park, Euwie; Parsons, Ella; Oravitan, Philip; Chen, Matthew; Molina, Cristian; Richmond, Caleb; Reddi, Adith; Huang, Jason; Shugrue, Cooper; Coviello, Rose; Unver, Selma; Indelicarto, Matthew; Islamovic, Emir; McIlroy, Rosemary; Yang, Alana; Hamad, Mahdi; Griffin, Elizabeth; Ahmed, Zara; Alla, Asha.
Afiliación
  • Kim ES; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Rajan A; Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Chang K; Department of Developmental Biology, Stanford University School of Medicine, Stanford, CA 94305, USA.
  • Govindarajan S; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Gulick C; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • English E; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Rodriguez B; Pritzker College Prep, Chicago, IL 60639, USA.
  • Bloomfield O; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Nakada S; Dalton School, New York, NY 10128, USA.
  • Beard C; Haileybury School, Hertford SG13 7NU, UK.
  • O'Connor S; Commack High School, 1 Scholar Ln, Commack, NY 11725, USA.
  • Mastroianni S; Commack High School, 1 Scholar Ln, Commack, NY 11725, USA.
  • Downey E; Commack High School, 1 Scholar Ln, Commack, NY 11725, USA.
  • Feigenbaum M; Commack High School, 1 Scholar Ln, Commack, NY 11725, USA.
  • Tolentino C; Commack High School, 1 Scholar Ln, Commack, NY 11725, USA.
  • Pace A; Commack High School, 1 Scholar Ln, Commack, NY 11725, USA.
  • Khan M; Commack High School, 1 Scholar Ln, Commack, NY 11725, USA.
  • Moon S; Commack High School, 1 Scholar Ln, Commack, NY 11725, USA.
  • DiPrima J; Commack High School, 1 Scholar Ln, Commack, NY 11725, USA.
  • Syed A; Commack High School, 1 Scholar Ln, Commack, NY 11725, USA.
  • Lin F; Commack High School, 1 Scholar Ln, Commack, NY 11725, USA.
  • Abukhadra Y; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Bacon I; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Beckerle J; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Cho S; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Donkor NE; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Garberg L; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Harrington A; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Hoang M; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Lawani N; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Noori A; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Park E; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Parsons E; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Oravitan P; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Chen M; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Molina C; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Richmond C; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Reddi A; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Huang J; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Shugrue C; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Coviello R; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Unver S; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Indelicarto M; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Islamovic E; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • McIlroy R; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Yang A; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Hamad M; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Griffin E; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Ahmed Z; Phillips Exeter Academy, Exeter, NH 03833, USA.
  • Alla A; Phillips Exeter Academy, Exeter, NH 03833, USA.
G3 (Bethesda) ; 13(9)2023 08 30.
Article en En | MEDLINE | ID: mdl-37279923
Conditional gene regulation in Drosophila through binary expression systems like the LexA-LexAop system provides a superb tool for investigating gene and tissue function. To increase the availability of defined LexA enhancer trap insertions, we present molecular, genetic, and tissue expression studies of 301 novel Stan-X LexA enhancer traps derived from mobilization of the index SX4 line. This includes insertions into distinct loci on the X, II, and III chromosomes that were not previously associated with enhancer traps or targeted LexA constructs, an insertion into ptc, and seventeen insertions into natural transposons. A subset of enhancer traps was expressed in CNS neurons known to produce and secrete insulin, an essential regulator of growth, development, and metabolism. Fly lines described here were generated and characterized through studies by students and teachers in an international network of genetics classes at public, independent high schools, and universities serving a diversity of students, including those underrepresented in science. Thus, a unique partnership between secondary schools and university-based programs has produced and characterized novel resources in Drosophila, establishing instructional paradigms devoted to unscripted experimental science.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Drosophila / Drosophila Límite: Animals Idioma: En Revista: G3 (Bethesda) Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Drosophila / Drosophila Límite: Animals Idioma: En Revista: G3 (Bethesda) Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido