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UHPLC-Q-Orbitrap HR-MS-Based Metabolomics for Profiling the Sida rhombifolia Metabolites with Different Plant Organs and Cultivation Ages.
Silviani, Dea; Zam Astari, Safirah; Anggraini Septianingsih, Dewi; Hudatul Karomah, Alfi; Ilmiawati, Auliya; Dyah Syafitri, Utami; Tri Wahyuni, Wulan; Siti Aminah, Nanik; Insanu, Muhamad; Rohman, Abdul; Rafi, Mohamad.
Afiliación
  • Silviani D; Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Tanjung Kampus, IPB Dramaga, Bogor, 16680, Indonesia.
  • Zam Astari S; Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Tanjung Kampus, IPB Dramaga, Bogor, 16680, Indonesia.
  • Anggraini Septianingsih D; Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Tanjung Kampus, IPB Dramaga, Bogor, 16680, Indonesia.
  • Hudatul Karomah A; Advanced Research Laboratory - Institute of Research and Community Service, IPB University, Jalan Palem Raya, Kampus IPB Dramaga, Bogor, 16680, Indonesia.
  • Ilmiawati A; Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Tanjung Kampus, IPB Dramaga, Bogor, 16680, Indonesia.
  • Dyah Syafitri U; Advanced Research Laboratory - Institute of Research and Community Service, IPB University, Jalan Palem Raya, Kampus IPB Dramaga, Bogor, 16680, Indonesia.
  • Tri Wahyuni W; Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Tanjung Kampus, IPB Dramaga, Bogor, 16680, Indonesia.
  • Siti Aminah N; Tropical Biopharmaca Research Center, Institute of Research and Community Services, IPB University, Jl. Taman Kencana No. 3, Bogor, 16128, Indonesia.
  • Insanu M; Tropical Biopharmaca Research Center, Institute of Research and Community Services, IPB University, Jl. Taman Kencana No. 3, Bogor, 16128, Indonesia.
  • Rohman A; Department of Statistics, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Meranti kampus IPB Dramaga, Bogor, 16680, Indonesia.
  • Rafi M; Department of Chemistry, Faculty of Mathematics and Natural Sciences, IPB University, Jl. Tanjung Kampus, IPB Dramaga, Bogor, 16680, Indonesia.
Chem Biodivers ; 20(6): e202201042, 2023 Jun.
Article en En | MEDLINE | ID: mdl-37276379
Plant organs and cultivation ages can result in different compositions and concentration levels of plant metabolites. The metabolite profile of plants can be determined using liquid chromatography. This study determined the metabolite profiles of leaves, stems, and roots of Sida rhombifolia at different cultivation ages at 3, 4, and 5 months post-planting (MPP) using liquid chromatography-mass spectrometry/mass spectrometry (LC/MS/MS). The results identified that 41 metabolites in S. rhombifolia extract for all plant organs and cultivation ages. We successfully identified approximately 36 (leaves), 22 (stems), and 18 (roots) compounds in all extract. Using principal component analysis (PCA) with peak area as the variable, we clustered all sample extracts based on plant organs and cultivation ages. As a result of PCA, S. rhombifolia extracts were grouped according to plant organs and cultivation ages. In conclusion, a clear difference in the composition and concentration levels of metabolites was observed in the leaves, stems, and roots of S. rhombifolia harvested at 3-, 4-, and 5-MPP.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masas en Tándem / Metabolómica Tipo de estudio: Prognostic_studies Idioma: En Revista: Chem Biodivers Asunto de la revista: BIOQUIMICA / QUIMICA Año: 2023 Tipo del documento: Article País de afiliación: Indonesia Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Espectrometría de Masas en Tándem / Metabolómica Tipo de estudio: Prognostic_studies Idioma: En Revista: Chem Biodivers Asunto de la revista: BIOQUIMICA / QUIMICA Año: 2023 Tipo del documento: Article País de afiliación: Indonesia Pais de publicación: Suiza