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Towards a model of biliary atresia - Pilot feasibility study in newborn piglets.
Helt, Thora Wesenberg; Buelund, Lene; Borgwardt, Lise; Eriksen, Thomas; Johansen, Lars; de Nijs, Robin; Holm, Soren; Burrin, Douglas G; Thymann, Thomas; Christensen, Vibeke Brix.
Afiliación
  • Helt TW; Department of Clinical Physiology and Nuclear Medicine, Rigshospitalet, Copenhagen University Hospital, Denmark.
  • Buelund L; Department of Veterinary Clinical Sciences, University of Copenhagen, Denmark.
  • Borgwardt L; Department of Clinical Physiology and Nuclear Medicine, Rigshospitalet, Copenhagen University Hospital, Denmark.
  • Eriksen T; Department of Veterinary Clinical Sciences, University of Copenhagen, Denmark.
  • Johansen L; Department of Pediatrics, Rigshospitalet, Copenhagen University Hospital, Denmark.
  • de Nijs R; Department of Clinical Physiology and Nuclear Medicine, Rigshospitalet, Copenhagen University Hospital, Denmark.
  • Holm S; Department of Clinical Physiology and Nuclear Medicine, Rigshospitalet, Copenhagen University Hospital, Denmark.
  • Burrin DG; Childrens Nutrition Research Center, Baylor College of Med, TX, USA.
  • Thymann T; Department of Veterinary and Animal Science, University of Copenhagen, Denmark.
  • Christensen VB; Department of Pediatrics, Rigshospitalet, Copenhagen University Hospital, Denmark.
Biochem Biophys Rep ; 34: 101487, 2023 Jul.
Article en En | MEDLINE | ID: mdl-37265596
Biliary atresia (BA) is a rare congenital liver disease with unknown etiology, and it is the most common indication for liver transplantation in children. As BA infants suffer from intestinal malabsorption and neurodevelopmental deficits, it is necessary to identify optimal medical and nutritional strategies using appropriate neonatal animal models. We aim to determine the feasibility of using newborn piglets with surgically induced cholestasis (bile duct ligation (BDL)) to mimic clinical features of BA. Six piglets were subjected to abdominal surgery on day 4 after birth. The bile ducts were ligated, and the piglet were followed for up to 12 days. On day 12 the piglets were subjected to a hepatobiliary scintigraphy using the tracer radiolabeled Technetium(99m-tc)-mebrofenin, and blood samples were collected for biochemical profiling. Of the six piglets, hepatobiliary scintigraphy verified that two piglets (BDL) had no excretion of bile into the duodenum, i.e. full cholestasis with a hepatic extraction fraction of 84-87% and clearance time of 230-318 min. One piglet (SHAM) had bile excretion to the duodenum. In accordance with this, the BDL piglets had steatorrhea, and increased levels of bilirubin and gammaglutamyl transferase (GGT). The last three piglets were euthanized due to bile leakage or poor growth. Surgically induced cholestasis in young piglets, may offer an animal model that displays clinical characteristics of biliary atresia, including malabsorption, hyperbilirubinaemia, increased GGT and reduced hepatic excretory function. Following refinement, this animal model may be used to optimize feeding strategies to secure optimal nutrition and neurodevelopment for neonatal cholestasis/BA patients.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Biochem Biophys Rep Año: 2023 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Biochem Biophys Rep Año: 2023 Tipo del documento: Article País de afiliación: Dinamarca Pais de publicación: Países Bajos