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Metabolomics of bacterial-fungal pairwise interactions reveal conserved molecular mechanisms.
Luu, Gordon T; Little, Jessica C; Pierce, Emily C; Morin, Manon; Ertekin, Celine A; Wolfe, Benjamin E; Baars, Oliver; Dutton, Rachel J; Sanchez, Laura M.
Afiliación
  • Luu GT; Department of Chemistry and Biochemistry, University of California Santa Cruz, Santa Cruz, California, 95064, USA. lmsanche@ucsc.edu.
  • Little JC; Department of Pharmaceutical Sciences, University of Illinois at Chicago, Chicago, Illinois, 60612, USA.
  • Pierce EC; Division of Biological Sciences, University of California San Diego, La Jolla, California, 92093, USA.
  • Morin M; Division of Biological Sciences, University of California San Diego, La Jolla, California, 92093, USA.
  • Ertekin CA; Department of Chemistry and Biochemistry, University of California Santa Cruz, Santa Cruz, California, 95064, USA. lmsanche@ucsc.edu.
  • Wolfe BE; Department of Biology, Tufts University, Medford, Massachusetts, 02155, USA.
  • Baars O; Tufts University Sensory and Science Center, Medford, Massachusetts, 02155, USA.
  • Dutton RJ; Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, North Carolina, 27607, USA.
  • Sanchez LM; Division of Biological Sciences, University of California San Diego, La Jolla, California, 92093, USA.
Analyst ; 148(13): 3002-3018, 2023 Jun 26.
Article en En | MEDLINE | ID: mdl-37259951
Bacterial-fungal interactions (BFIs) can shape the structure of microbial communities, but the small molecules mediating these BFIs are often understudied. We explored various optimization steps for our microbial culture and chemical extraction protocols for bacterial-fungal co-cultures, and liquid chromatography-tandem mass spectrometry (LC-MS/MS) revealed that metabolomic profiles are mainly comprised of fungi derived features, indicating that fungi are the key contributors to small molecules in BFIs. LC-inductively coupled plasma MS (LC-ICP-MS) and MS/MS based dereplication using database searching revealed the presence of several known fungal specialized metabolites and structurally related analogues in these extracts, including siderophores such as desferrichrome, desferricoprogen, and palmitoylcoprogen. Among these analogues, a novel putative coprogen analogue possessing a terminal carboxylic acid motif was identified from Scopulariopsis sp. JB370, a common cheese rind fungus, and its structure was elucidated via MS/MS fragmentation. Based on these findings, filamentous fungal species appear to be capable of producing multiple siderophores with potentially different biological roles (i.e. various affinities for different forms of iron). These findings highlight that fungal species are important contributors to microbiomes via their production of abundant specialized metabolites and that elucidating their role in complex communities should continue to be a priority.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sideróforos / Espectrometría de Masas en Tándem Tipo de estudio: Prognostic_studies Idioma: En Revista: Analyst Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sideróforos / Espectrometría de Masas en Tándem Tipo de estudio: Prognostic_studies Idioma: En Revista: Analyst Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido