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Multiple accurate and sensitive arrays for Capripoxvirus (CaPV) differentiation.
Cao, Gaihua; Xiong, Yifan; Shi, Meimei; Qiu, Yue; Wang, Yu; Nie, Fuping; Huo, Danqun; Hou, Changjun.
Afiliación
  • Cao G; Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China.
  • Xiong Y; Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China.
  • Shi M; State Key Laboratory of Cattle Diseases Detection (Chongqing) of Customs, Diagnosis and Testing Laboratory of Lumpy Skin Disease, Chongqing Customs Technology Center, Chongqing, 400020, PR China.
  • Qiu Y; Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China.
  • Wang Y; State Key Laboratory of Cattle Diseases Detection (Chongqing) of Customs, Diagnosis and Testing Laboratory of Lumpy Skin Disease, Chongqing Customs Technology Center, Chongqing, 400020, PR China.
  • Nie F; State Key Laboratory of Cattle Diseases Detection (Chongqing) of Customs, Diagnosis and Testing Laboratory of Lumpy Skin Disease, Chongqing Customs Technology Center, Chongqing, 400020, PR China. Electronic address: nie1626@163.com.
  • Huo D; Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China. Electronic address: huodq@cqu.edu.cn.
  • Hou C; Key Laboratory for Biorheological Science and Technology of Ministry of Education, Bioengineering College of Chongqing University, Chongqing, 400044, PR China; Chongqing Key Laboratory of Bio-perception & Intelligent Information Processing, School of Microelectronics and Communication Engineerin
Anal Chim Acta ; 1267: 341391, 2023 Aug 01.
Article en En | MEDLINE | ID: mdl-37257965
Capripoxvirus (CaPV) contains three viruses that have caused massive losses in the livestock and dairy industries. Accurate CaPV differentiation has far-reaching implications for effectively controlling outbreaks. However, it has a great challenge to distinguishing three viruses due to high homology of 97%. Here, we established a sensitive CRISPR/Cas12a array based on Multiple-recombinase polymerase amplification (M-RPA) for CaPV differentiation, which provided a more comprehensive and accurate differentiation mode targeting VARV B22R and RPO30 genes. By sensitive CRISPR/Cas12a and M-RPA, the actual detection limits of three viruses were as low as 50, 40 and 60 copies, respectively. Moreover, Lateral flow dipstick (LFD) array based on CRISPR/Cas12a achieved portable and intuitive detection, making it suitable for point-of-care testing. Therefore, CRISPR/Cas12a array and LFD array paved the way for CaPV differentiation in practice. Additionally, we constructed a real-time quantitative PCR (qPCR) array to fill the qPCR technical gap in differentiation and to facilitate the quarantine departments.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Capripoxvirus / Infecciones por Poxviridae Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Anal Chim Acta Año: 2023 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Capripoxvirus / Infecciones por Poxviridae Tipo de estudio: Diagnostic_studies Límite: Animals Idioma: En Revista: Anal Chim Acta Año: 2023 Tipo del documento: Article Pais de publicación: Países Bajos