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The effect of Cissus quadrangularis Salisb. extract on maturation of rat mesenchymal stem cells.
Eriani, K; Desriani, D; Putri, Vianti C; Nursanty, R; Mariya, S; Umaratusalihah, S; Ichsan, I; Saidi, N.
Afiliación
  • Eriani K; Universitas Syiah Kuala, Faculty of Mathematics and Natural Sciences, Department of Biology, Banda Aceh, Indonesia.
  • Desriani D; Research Center for Genetic Engineering National Research and Innovation Agency, Cibinong, Indonesia.
  • Putri VC; Universitas Syiah Kuala, Faculty of Mathematics and Natural Sciences, Department of Biology, Banda Aceh, Indonesia.
  • Nursanty R; Universitas Syiah Kuala, Faculty of Mathematics and Natural Sciences, Department of Biology, Banda Aceh, Indonesia.
  • Mariya S; IPB University, Primate Research Center, Bogor, Indonesia.
  • Umaratusalihah S; Universitas Syiah Kuala, Faculty of Mathematics and Natural Sciences, Department of Biology, Banda Aceh, Indonesia.
  • Ichsan I; Universitas Syiah Kuala, Faculty of Medicine, Banda Aceh, Aceh, Indonesia.
  • Saidi N; Universitas Syiah Kuala, Department of Chemistry, Banda Aceh, Aceh, Indonesia.
Braz J Biol ; 83: e270335, 2023.
Article en En | MEDLINE | ID: mdl-37255198
Degenerative diseases, such as osteoporosis, could be treated by stem cells. The aim of this study was to identify the gene expression of bone marrow mesenchymal stem cells (BM-MSC) derived from Sprague Dawley rats and to assess the effect of Cissus quadrangularis Salisb. extract on their maturation into bone cells. The BM-MSC were divided into three groups: (a) BM-MSCs + osteoblast cell growth basal medium as the positive control; (b) BM-MSCs + Dulbecco's modified eagle's medium (DMEM) + 0.3 mg/mL methanol extract of C. quadrangularis as methanol group; and (c) BM-MSC + DMEM + 0.3 mg/mL ethyl acetate extract of C. quadrangularis as ethyl acetate group. A relative quantification approach using was used to analyze the expression of the alp (alkaline phosphatase) gene, with the beta-actin gene was used to normalize the expression of the alp gene. The intra-assay variation was calculated to validate the RT-qPCR data. Our study found that the intra-assay variation value was acceptable, with most of the coefficients of variability (CV) value <5. Ethyl acetate solvent outperformed methanol solvent in extracting the active compound C. quadrangularis. In the ethyl acetate extract group, the expression of the alp gene increased three times compared to the positive control. In methanol extract group, the expression of alp gene was lower six times compared to positive control. This study suggests that C. quadrangularis extracts using ethyl acetate could induce the maturation of BM-MSCs. However, further studies are warrant to confirm this effect using different indicators.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cissus / Células Madre Mesenquimatosas Límite: Animals Idioma: En Revista: Braz J Biol Asunto de la revista: BIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Indonesia Pais de publicación: Brasil
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Cissus / Células Madre Mesenquimatosas Límite: Animals Idioma: En Revista: Braz J Biol Asunto de la revista: BIOLOGIA Año: 2023 Tipo del documento: Article País de afiliación: Indonesia Pais de publicación: Brasil