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Novel antiosteoporotic peptides purified from protein hydrolysates of taihe black-boned silky fowl: By larval zebrafish model and molecular docking.
Qiu, Yang; Ying, Jianyue; Yan, Fujie; Yu, Huilin; Zhao, Yan; Li, Honghao; Xia, Shengyao; Chen, Jianchu; Zhu, Jiajin.
Afiliación
  • Qiu Y; College of Biosystems Engineering & Food Science, Zhejiang University, Hangzhou 310058, China.
  • Ying J; Zhejiang University Hospital, Hangzhou 310027, China.
  • Yan F; College of Biosystems Engineering & Food Science, Zhejiang University, Hangzhou 310058, China.
  • Yu H; College of Biosystems Engineering & Food Science, Zhejiang University, Hangzhou 310058, China.
  • Zhao Y; College of Biosystems Engineering & Food Science, Zhejiang University, Hangzhou 310058, China.
  • Li H; College of Biosystems Engineering & Food Science, Zhejiang University, Hangzhou 310058, China.
  • Xia S; College of Biosystems Engineering & Food Science, Zhejiang University, Hangzhou 310058, China.
  • Chen J; College of Biosystems Engineering & Food Science, Zhejiang University, Hangzhou 310058, China. Electronic address: jc@zju.edu.cn.
  • Zhu J; College of Biosystems Engineering & Food Science, Zhejiang University, Hangzhou 310058, China; Zhejiang Engineering Center for Food Technology and Equipment, Zhejiang University, Hangzhou 310058, China; Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou 310058, China
Food Res Int ; 169: 112850, 2023 07.
Article en En | MEDLINE | ID: mdl-37254422
The black-boned silky fowl (BSF) muscle protein hydrolysate was gained by alcalase. The hydrolysate could stimulate MC3T3-E1 cell proliferation, as well as enhance alkaline phosphatas (ALP) activity and deposits of minerals. After isolation and purification, 55 peptide sequences with Mascot score over 40 were identified. Combined with molecular docking simulation and molecular dynamics analysis, two novel peptides (PASTGAAK and PGPPGTPF) were identified with the lowest binding energy of -4.99 kcal/mol and -3.07 kcal/mol with receptor BMPR1A of BMP-2/Smad pathway, showing the ability to increase BMPR1A stability. Moreover, both PASTGAAK and PGPPGTPF revealed strong anti-osteoporosis activities in the zebrafish model induced by dexamethasone. Additionally, the identified peptides could be beneficial for the differentiation of MC3T3-E1 cell for upregulating the expression of some osteoblast-related genes and proteins by stimulating BMP-2/Smad pathway. Overall, the two newly identified peptides could be the potential candidate to prevent osteoporosis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hidrolisados de Proteína / Pez Cebra Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Food Res Int Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Canadá
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Hidrolisados de Proteína / Pez Cebra Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Food Res Int Año: 2023 Tipo del documento: Article País de afiliación: China Pais de publicación: Canadá