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Impact of Needle Selection on Survival of Muscle-Derived Cells When Used for Laryngeal Injections.
Awonusi, Oluwaseyi; Harbin, Zachary J; Brookes, Sarah; Zhang, Lujuan; Kaefer, Samuel; Morrison, Rachel A; Newman, Sharlé; Voytik-Harbin, Sherry; Halum, Stacey.
Afiliación
  • Awonusi O; Department of Otolaryngology-Head and Neck Surgery, Indiana University School of Medicine (IUSM), Indianapolis, IN, USA.
  • Harbin ZJ; School of Mechanical Engineering, Purdue University, West Lafayette, IN, USA.
  • Brookes S; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA.
  • Zhang L; Department of Otolaryngology-Head and Neck Surgery, Indiana University School of Medicine (IUSM), Indianapolis, IN, USA.
  • Kaefer S; Department of Otolaryngology-Head and Neck Surgery, Indiana University School of Medicine (IUSM), Indianapolis, IN, USA.
  • Morrison RA; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA.
  • Newman S; Department of Otolaryngology-Head and Neck Surgery, Indiana University School of Medicine (IUSM), Indianapolis, IN, USA.
  • Voytik-Harbin S; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN, USA.
  • Halum S; Department of Basic Medical Sciences, Purdue University, West Lafayette, IN, USA.
J Cell Sci Ther ; 14(1)2023.
Article en En | MEDLINE | ID: mdl-37250272
Objective: To describe how differing injector needles and delivery vehicles impact Autologous Muscle-Derived Cell (AMDC) viability when used for laryngeal injection. Methods: In this study, adult porcine muscle tissue was harvested and used to create AMDC populations. While controlling cell concentration (1 × 107 cells/ml), AMDCs including Muscle Progenitor Cells (MPCs) or Motor Endplate Expressing Cells (MEEs) were suspended in either phosphate-buffered saline or polymerizable (in-situ scaffold forming) type I oligomeric collagen solution. Cell suspensions were then injected through 23- and 27-gauge needles of different lengths at the same rate (2 ml/min) using a syringe pump. Cell viability was measured immediately after injection and 24- and 48-hours post-injection, and then compared to baseline cell viability prior to injection. Results: The viability of cells post-injection was not impacted by needle length or needle gauge but was significantly impacted by the delivery vehicle. Overall, injection of cells using collagen as a delivery vehicle maintained the highest cell viability. Conclusion: Needle gauge, needle length, and delivery vehicle are important factors that can affect the viability of injected cell populations. These factors should be considered and adapted to improve injectable MDC therapy outcomes when used for laryngeal applications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Cell Sci Ther Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Cell Sci Ther Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos