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Mutational analysis of the functional motifs of the DEAD-box RNA helicase Me31B/DDX6 in Drosophila germline development.
Kara, Evan; McCambridge, Aidan; Proffer, Megan; Dilts, Carol; Pumnea, Brooke; Eshak, John; Smith, Korey A; Fielder, Isaac; Doyle, Dominique A; Ortega, Bianca M; Mukatash, Yousif; Malik, Noor; Mohammed, Ammaar R; Govani, Deep; Niepielko, Matthew G; Gao, Ming.
Afiliación
  • Kara E; Biology Department, Indiana University Northwest, Gary, IN, USA.
  • McCambridge A; Biology Department, Indiana University Northwest, Gary, IN, USA.
  • Proffer M; Biology Department, Indiana University Northwest, Gary, IN, USA.
  • Dilts C; Biology Department, Indiana University Northwest, Gary, IN, USA.
  • Pumnea B; Biology Department, Indiana University Northwest, Gary, IN, USA.
  • Eshak J; Biology Department, Indiana University Northwest, Gary, IN, USA.
  • Smith KA; Biology Department, Indiana University Northwest, Gary, IN, USA.
  • Fielder I; Biology Department, Indiana University Northwest, Gary, IN, USA.
  • Doyle DA; School of Integrative Science and Technology, Kean University, Union, NJ, USA.
  • Ortega BM; School of Integrative Science and Technology, Kean University, Union, NJ, USA.
  • Mukatash Y; Biology Department, Indiana University Northwest, Gary, IN, USA.
  • Malik N; Biology Department, Indiana University Northwest, Gary, IN, USA.
  • Mohammed AR; Biology Department, Indiana University Northwest, Gary, IN, USA.
  • Govani D; Biology Department, Indiana University Northwest, Gary, IN, USA.
  • Niepielko MG; School of Integrative Science and Technology, Kean University, Union, NJ, USA.
  • Gao M; Biology Department, Kean University, Union, NJ, USA.
FEBS Lett ; 597(14): 1848-1867, 2023 07.
Article en En | MEDLINE | ID: mdl-37235728
Me31B/DDX6 is a DEAD-box family RNA helicase playing roles in post-transcriptional RNA regulation in different cell types and species. Despite the known motifs/domains of Me31B, the in vivo functions of the motifs remain unclear. Here, we used the Drosophila germline as a model and used CRISPR to mutate the key Me31B motifs/domains: helicase domain, N-terminal domain, C-terminal domain and FDF-binding motif. Then, we performed screening characterization on the mutants and report the effects of the mutations on the Drosophila germline, on processes such as fertility, oogenesis, embryo patterning, germline mRNA regulation and Me31B protein expression. The study indicates that the Me31B motifs contribute different functions to the protein and are needed for proper germline development, providing insights into the in vivo working mechanism of the helicase.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Drosophila / Drosophila Límite: Animals Idioma: En Revista: FEBS Lett Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas de Drosophila / Drosophila Límite: Animals Idioma: En Revista: FEBS Lett Año: 2023 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido