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Localized Surface Plasmon Resonance-Based Nanosensor for Rapid Detection of Glyphosate in Food Samples.
Côco, Ariany Soares; Campos, Fabiana Vasconcelos; Díaz, Camilo Arturo Rodríguez; Guimarães, Marco César Cunegundes; Prado, Adilson Ribeiro; de Oliveira, Jairo Pinto.
Afiliación
  • Côco AS; Functional Nanomaterials Laboratory, Morphology Department, Federal University of Espírito Santo (UFES), Av Marechal Campos 1468, Vitória 29040-090, ES, Brazil.
  • Campos FV; Functional Nanomaterials Laboratory, Morphology Department, Federal University of Espírito Santo (UFES), Av Marechal Campos 1468, Vitória 29040-090, ES, Brazil.
  • Díaz CAR; Telecommunications Laboratory, Electrical Engineering Department, Federal University of Espírito Santo (UFES), Av Fernando Ferrari 514, Vitória 29075-910, ES, Brazil.
  • Guimarães MCC; Functional Nanomaterials Laboratory, Morphology Department, Federal University of Espírito Santo (UFES), Av Marechal Campos 1468, Vitória 29040-090, ES, Brazil.
  • Prado AR; Federal Institute of Espírito Santo (IFES), km 6.5 ES 010, Vitória 29173-087, ES, Brazil.
  • de Oliveira JP; Functional Nanomaterials Laboratory, Morphology Department, Federal University of Espírito Santo (UFES), Av Marechal Campos 1468, Vitória 29040-090, ES, Brazil.
Biosensors (Basel) ; 13(5)2023 Apr 30.
Article en En | MEDLINE | ID: mdl-37232873
In this study, we developed a biosensor based on the localized surface plasmon resonance (LSPR) phenomenon of gold nanoparticles (AuNPs) to detect the widely used herbicide glyphosate in food samples. To do so, either cysteamine or a specific antibody for glyphosate were conjugated to the surface of the nanoparticles. AuNPs were synthesized using the sodium citrate reduction method and had their concentration determined via inductively plasma coupled mass spectrometry. Their optical properties were analyzed using UV-vis spectroscopy, X-ray diffraction, and transmission electron microscopy. Functionalized AuNPs were further characterized via Fourier-transform infrared spectroscopy, Raman scattering, Zeta potential, and dynamic light scattering. Both conjugates succeeded in detecting the presence of glyphosate in the colloid, although nanoparticles functionalized with cysteamine tended to aggregate at high concentrations of the herbicide. On the other hand, AuNPs functionalized with anti-glyphosate functioned at a broad concentration range and successfully identified the presence of the herbicide in non-organic coffee samples and when it was added to an organic coffee sample. This study demonstrates the potential of AuNP-based biosensors to detect glyphosate in food samples. The low-cost and specificity of these biosensors make them a viable alternative to current methods for detecting glyphosate in foodstuffs.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Resonancia por Plasmón de Superficie / Nanopartículas del Metal Tipo de estudio: Diagnostic_studies Idioma: En Revista: Biosensors (Basel) Año: 2023 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Resonancia por Plasmón de Superficie / Nanopartículas del Metal Tipo de estudio: Diagnostic_studies Idioma: En Revista: Biosensors (Basel) Año: 2023 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza