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Sequencing by avidity enables high accuracy with low reagent consumption.
Arslan, Sinan; Garcia, Francisco J; Guo, Minghao; Kellinger, Matthew W; Kruglyak, Semyon; LeVieux, Jake A; Mah, Adeline H; Wang, Haosen; Zhao, Junhua; Zhou, Chunhong; Altomare, Andrew; Bailey, John; Byrne, Matthew B; Chang, Chiting; Chen, Steve X; Cho, Byungrae; Dennler, Claudia N; Dien, Vivian T; Fuller, Derek; Kelley, Ryan; Khandan, Omid; Klein, Michael G; Kim, Michael; Lajoie, Bryan R; Lin, Bill; Liu, Yu; Lopez, Tyler; Mains, Peter T; Price, Andrew D; Robertson, Samantha R; Taylor-Weiner, Hermes; Tippana, Ramreddy; Tomaney, Austin B; Zhang, Su; Abtahi, Minna; Ambroso, Mark R; Bajari, Rosita; Bellizzi, Ava M; Benitez, Chris B; Berard, Daniel R; Berti, Lorenzo; Blease, Kelly N; Blum, Angela P; Boddicker, Andrew M; Bondar, Leo; Brown, Chris; Bui, Chris A; Calleja-Aguirre, Juan; Cappa, Kevin; Chan, Joshua.
Afiliación
  • Arslan S; Element Biosciences, San Diego, CA, USA.
  • Garcia FJ; Element Biosciences, San Diego, CA, USA.
  • Guo M; Element Biosciences, San Diego, CA, USA.
  • Kellinger MW; Element Biosciences, San Diego, CA, USA.
  • Kruglyak S; Element Biosciences, San Diego, CA, USA.
  • LeVieux JA; Element Biosciences, San Diego, CA, USA.
  • Mah AH; Element Biosciences, San Diego, CA, USA.
  • Wang H; Element Biosciences, San Diego, CA, USA.
  • Zhao J; Element Biosciences, San Diego, CA, USA.
  • Zhou C; Element Biosciences, San Diego, CA, USA.
  • Altomare A; Element Biosciences, San Diego, CA, USA.
  • Bailey J; Element Biosciences, San Diego, CA, USA.
  • Byrne MB; Element Biosciences, San Diego, CA, USA.
  • Chang C; Element Biosciences, San Diego, CA, USA.
  • Chen SX; Element Biosciences, San Diego, CA, USA.
  • Cho B; Element Biosciences, San Diego, CA, USA.
  • Dennler CN; Element Biosciences, San Diego, CA, USA.
  • Dien VT; Element Biosciences, San Diego, CA, USA.
  • Fuller D; Element Biosciences, San Diego, CA, USA.
  • Kelley R; Element Biosciences, San Diego, CA, USA.
  • Khandan O; Element Biosciences, San Diego, CA, USA.
  • Klein MG; Element Biosciences, San Diego, CA, USA.
  • Kim M; Element Biosciences, San Diego, CA, USA.
  • Lajoie BR; Element Biosciences, San Diego, CA, USA.
  • Lin B; Element Biosciences, San Diego, CA, USA.
  • Liu Y; Element Biosciences, San Diego, CA, USA.
  • Lopez T; Element Biosciences, San Diego, CA, USA.
  • Mains PT; Element Biosciences, San Diego, CA, USA.
  • Price AD; Element Biosciences, San Diego, CA, USA.
  • Robertson SR; Element Biosciences, San Diego, CA, USA.
  • Taylor-Weiner H; Element Biosciences, San Diego, CA, USA.
  • Tippana R; Element Biosciences, San Diego, CA, USA.
  • Tomaney AB; Element Biosciences, San Diego, CA, USA.
  • Zhang S; Element Biosciences, San Diego, CA, USA.
  • Abtahi M; Element Biosciences, San Diego, CA, USA.
  • Ambroso MR; Element Biosciences, San Diego, CA, USA.
  • Bajari R; Element Biosciences, San Diego, CA, USA.
  • Bellizzi AM; Element Biosciences, San Diego, CA, USA.
  • Benitez CB; Element Biosciences, San Diego, CA, USA.
  • Berard DR; Element Biosciences, San Diego, CA, USA.
  • Berti L; Element Biosciences, San Diego, CA, USA.
  • Blease KN; Element Biosciences, San Diego, CA, USA.
  • Blum AP; Element Biosciences, San Diego, CA, USA.
  • Boddicker AM; Element Biosciences, San Diego, CA, USA.
  • Bondar L; Element Biosciences, San Diego, CA, USA.
  • Brown C; Element Biosciences, San Diego, CA, USA.
  • Bui CA; Element Biosciences, San Diego, CA, USA.
  • Calleja-Aguirre J; Element Biosciences, San Diego, CA, USA.
  • Cappa K; Element Biosciences, San Diego, CA, USA.
  • Chan J; Element Biosciences, San Diego, CA, USA.
Nat Biotechnol ; 42(1): 132-138, 2024 Jan.
Article en En | MEDLINE | ID: mdl-37231263
We present avidity sequencing, a sequencing chemistry that separately optimizes the processes of stepping along a DNA template and that of identifying each nucleotide within the template. Nucleotide identification uses multivalent nucleotide ligands on dye-labeled cores to form polymerase-polymer-nucleotide complexes bound to clonal copies of DNA targets. These polymer-nucleotide substrates, termed avidites, decrease the required concentration of reporting nucleotides from micromolar to nanomolar and yield negligible dissociation rates. Avidity sequencing achieves high accuracy, with 96.2% and 85.4% of base calls having an average of one error per 1,000 and 10,000 base pairs, respectively. We show that the average error rate of avidity sequencing remained stable following a long homopolymer.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Nucleótidos Idioma: En Revista: Nat Biotechnol Asunto de la revista: BIOTECNOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
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PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN / Nucleótidos Idioma: En Revista: Nat Biotechnol Asunto de la revista: BIOTECNOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos