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Human oral lectin ZG16B acts as a cell wall polysaccharide probe to decode host-microbe interactions with oral commensals.
Ghosh, Soumi; Ahearn, Christian P; Isabella, Christine R; Marando, Victoria M; Dodge, Gregory J; Bartlett, Helen; McPherson, Robert L; Dugan, Amanda E; Jain, Shikha; Neznanova, Lubov; Tettelin, Hervé; Putnik, Rachel; Grimes, Catherine L; Ruhl, Stefan; Kiessling, Laura L; Imperiali, Barbara.
Afiliación
  • Ghosh S; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139.
  • Ahearn CP; Department of Oral Biology, University at Buffalo School of Dental Medicine, Buffalo, NY 14214.
  • Isabella CR; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139.
  • Marando VM; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139.
  • Dodge GJ; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139.
  • Bartlett H; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139.
  • McPherson RL; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139.
  • Dugan AE; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139.
  • Jain S; Department of Oral Biology, University at Buffalo School of Dental Medicine, Buffalo, NY 14214.
  • Neznanova L; Department of Oral Biology, University at Buffalo School of Dental Medicine, Buffalo, NY 14214.
  • Tettelin H; Department of Microbiology and Immunology, Institute for Genome Sciences, University of Maryland School of Medicine, Baltimore, MD 21201.
  • Putnik R; Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716.
  • Grimes CL; Department of Chemistry and Biochemistry, University of Delaware, Newark, DE 19716.
  • Ruhl S; Department of Oral Biology, University at Buffalo School of Dental Medicine, Buffalo, NY 14214.
  • Kiessling LL; Department of Chemistry, Massachusetts Institute of Technology, Cambridge, MA 02139.
  • Imperiali B; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139.
Proc Natl Acad Sci U S A ; 120(22): e2216304120, 2023 05 30.
Article en En | MEDLINE | ID: mdl-37216558
The oral microbiome is critical to human health and disease, yet the role that host salivary proteins play in maintaining oral health is unclear. A highly expressed gene in human salivary glands encodes the lectin zymogen granule protein 16 homolog B (ZG16B). Despite the abundance of this protein, its interaction partners in the oral microbiome are unknown. ZG16B possesses a lectin fold, but whether it binds carbohydrates is unclear. We postulated that ZG16B would bind microbial glycans to mediate recognition of oral microbes. To this end, we developed a microbial glycan analysis probe (mGAP) strategy based on conjugating the recombinant protein to fluorescent or biotin reporter functionality. Applying the ZG16B-mGAP to dental plaque isolates revealed that ZG16B predominantly binds to a limited set of oral microbes, including Streptococcus mitis, Gemella haemolysans, and, most prominently, Streptococcus vestibularis. S. vestibularis is a commensal bacterium widely distributed in healthy individuals. ZG16B binds to S. vestibularis through the cell wall polysaccharides attached to the peptidoglycan, indicating that the protein is a lectin. ZG16B slows the growth of S. vestibularis with no cytotoxicity, suggesting that it regulates S. vestibularis abundance. The mGAP probes also revealed that ZG16B interacts with the salivary mucin MUC7. Analysis of S. vestibularis and MUC7 with ZG16B using super-resolution microscopy supports ternary complex formation that can promote microbe clustering. Together, our data suggest that ZG16B influences the compositional balance of the oral microbiome by capturing commensal microbes and regulating their growth using a mucin-assisted clearance mechanism.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos y Proteínas de Señalización Intercelular / Interacciones Microbiota-Huesped / Lectinas Límite: Humans Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Péptidos y Proteínas de Señalización Intercelular / Interacciones Microbiota-Huesped / Lectinas Límite: Humans Idioma: En Revista: Proc Natl Acad Sci U S A Año: 2023 Tipo del documento: Article Pais de publicación: Estados Unidos