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Label-free intratissue activity imaging of alveolar organoids with dynamic optical coherence tomography.
Morishita, Rion; Suzuki, Toshio; Mukherjee, Pradipta; Abd El-Sadek, Ibrahim; Lim, Yiheng; Lichtenegger, Antonia; Makita, Shuichi; Tomita, Kiriko; Yamamoto, Yuki; Nagamoto, Tetsuharu; Yasuno, Yoshiaki.
Afiliación
  • Morishita R; Computational Optics Group, University of Tsukuba, Tsukuba, Ibaraki 305-8573, Japan.
  • Suzuki T; Department of Medical Oncology, Faculty of Medicine, University of Tsukuba, Ibaraki 305-8575, Japan.
  • Mukherjee P; HiLung Inc., Kyoto, Japan.
  • Abd El-Sadek I; Computational Optics Group, University of Tsukuba, Tsukuba, Ibaraki 305-8573, Japan.
  • Lim Y; Computational Optics Group, University of Tsukuba, Tsukuba, Ibaraki 305-8573, Japan.
  • Lichtenegger A; Department of Physics, Faculty of Science, Damietta University, New Damietta City 34517, Damietta, Egypt.
  • Makita S; Computational Optics Group, University of Tsukuba, Tsukuba, Ibaraki 305-8573, Japan.
  • Tomita K; Computational Optics Group, University of Tsukuba, Tsukuba, Ibaraki 305-8573, Japan.
  • Yamamoto Y; Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Währinger Gürtel 18-20, 4L, 1090, Vienna, Austria.
  • Nagamoto T; Computational Optics Group, University of Tsukuba, Tsukuba, Ibaraki 305-8573, Japan.
  • Yasuno Y; Computational Optics Group, University of Tsukuba, Tsukuba, Ibaraki 305-8573, Japan.
Biomed Opt Express ; 14(5): 2333-2351, 2023 May 01.
Article en En | MEDLINE | ID: mdl-37206117
An organoid is a three-dimensional (3D) in vitro cell culture emulating human organs. We applied 3D dynamic optical coherence tomography (DOCT) to visualize the intratissue and intracellular activities of human induced pluripotent stem cells (hiPSCs)-derived alveolar organoids in normal and fibrosis models. 3D DOCT data were acquired with an 840-nm spectral domain optical coherence tomography with axial and lateral resolutions of 3.8 µm (in tissue) and 4.9 µm, respectively. The DOCT images were obtained by the logarithmic-intensity-variance (LIV) algorithm, which is sensitive to the signal fluctuation magnitude. The LIV images revealed cystic structures surrounded by high-LIV borders and mesh-like structures with low LIV. The former may be alveoli with a highly dynamics epithelium, while the latter may be fibroblasts. The LIV images also demonstrated the abnormal repair of the alveolar epithelium.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomed Opt Express Año: 2023 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Biomed Opt Express Año: 2023 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos