Your browser doesn't support javascript.
loading
Protocol for the Investigation of Plasma and Whole Blood Clot Property of Fibrin Fiber Thickness Using Scanning Electron Microscopy.
Cai, Tengyi; McCafferty, Conor; Van Den Helm, Suelyn; Letunica, Natasha; Attard, Chantal; Monagle, Paul; Ignjatovic, Vera.
Afiliación
  • Cai T; Murdoch Children's Research Institute, Parkville, Australia.
  • McCafferty C; Department of Paediatrics, The University of Melbourne, Parkville, Australia.
  • Van Den Helm S; Murdoch Children's Research Institute, Parkville, Australia.
  • Letunica N; Department of Paediatrics, The University of Melbourne, Parkville, Australia.
  • Attard C; Murdoch Children's Research Institute, Parkville, Australia.
  • Monagle P; Department of Paediatrics, The University of Melbourne, Parkville, Australia.
  • Ignjatovic V; Murdoch Children's Research Institute, Parkville, Australia.
Methods Mol Biol ; 2663: 775-786, 2023.
Article en En | MEDLINE | ID: mdl-37204752
Blood clot formation represents a key component of the coagulation process for preventing excessive hemorrhage. The structural characteristics of blood clots are associated with their strength and susceptibility to fibrinolysis. Scanning electron microscopy is a technique that allows for state-of-the-art image capture of blood clots, providing visualization of topography, fibrin thickness, fibrin network density, and blood cell involvement and morphology. In this chapter, we provide a detailed protocol for characterization of plasma and whole blood clot structure using SEM, covering the spectrum from blood collection, in vitro clot formation, sample preparation for SEM, imaging, and image analysis, specifically focusing on the measurement of fibrin fiber thickness.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trombosis / Fibrina Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2023 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Trombosis / Fibrina Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2023 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos