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Oviduct epithelial cell­derived extracellular vesicles promote the developmental competence of IVF porcine embryos.
Fang, Xun; Bang, Seonggyu; Tanga, Bereket Molla; Seo, Chaerim; Zhou, Dongjie; Seong, Gyeonghwan; Saadeldin, Islam M; Lee, Sanghoon; Cui, Xiang-Shun; Cho, Jongki.
Afiliación
  • Fang X; Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea.
  • Bang S; Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea.
  • Tanga BM; Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea.
  • Seo C; Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea.
  • Zhou D; Department of Animal Sciences, Chungbuk National University, Cheongju 28644, Republic of Korea.
  • Seong G; Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea.
  • Saadeldin IM; Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea.
  • Lee S; Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea.
  • Cui XS; Department of Animal Sciences, Chungbuk National University, Cheongju 28644, Republic of Korea.
  • Cho J; Laboratory of Theriogenology, College of Veterinary Medicine, Chungnam National University, Daejeon 34134, Republic of Korea.
Mol Med Rep ; 27(6)2023 Jun.
Article en En | MEDLINE | ID: mdl-37203391
Assisted reproductive technology has increased the efficiency of animal reproduction. However, polyspermy is a significant limitation of porcine in vitro fertilization (IVF). Therefore, reducing the polyspermy rate and improving monospermic embryos is crucial. Recent studies have reported that oviductal fluid, along with its contents of extracellular vesicles (EVs), enhanced the fertilization process and supported embryo development. Consequently, the present study investigated the effects of porcine oviduct epithelial cells (OEC­EVs) on sperm­oocyte interactions during porcine IVF and evaluated in vitro embryo developmental competence outcomes. During IVF embryo development, the cleavage rate was significantly higher in the group treated with 50 ng/ml OEC­EVs compared with the control group (67.6±2.5 vs. 57.3±1.9; P<0.05). Furthermore, the OEC­EV group had significantly more embryos (16.4±1.2 vs. 10.2±0.8; P<0.05), and the polyspermy rate significantly decreased (32.9±2.5 vs. 43.8±3.1; P<0.05) compared with that of the control group. Additionally, the fluorescence intensities of cortical granules (3.56±0.47 vs. 2.15±0.24; P<0.05) and active mitochondria (8.14±0.34 vs. 5.96±0.38; P<0.05) were significantly higher in the OEC­EV group compared with those in the control group. In conclusion, OEC­EV adsorption and penetration crosstalk between sperm and oocytes was observed. OEC­EV treatment was demonstrated to significantly improve the concentration and distribution of cortical granules in oocytes. Furthermore, OEC­EVs also increased oocyte mitochondrial activity, reduced polyspermy and increased the IVF success rate.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Semen / Fertilización In Vitro Límite: Animals / Female / Humans / Male Idioma: En Revista: Mol Med Rep Año: 2023 Tipo del documento: Article Pais de publicación: Grecia
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Semen / Fertilización In Vitro Límite: Animals / Female / Humans / Male Idioma: En Revista: Mol Med Rep Año: 2023 Tipo del documento: Article Pais de publicación: Grecia