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Comparative sedimentation equilibrium analysis of two IgG1 glycoforms: IgGCri and IgGWid.
Abu Hammad, Khalil; Dinu, Vlad; MacCalman, Thomas E; Pattem, Jacob; Goodall, Margaret; Gillis, Richard B; Jefferis, Roy; Harding, Stephen E.
Afiliación
  • Abu Hammad K; National Centre for Macromolecular Hydrodynamics (NCMH), School of Biosciences, University of Nottingham, Sutton Bonington, LE12 5RD, UK.
  • Dinu V; National Centre for Macromolecular Hydrodynamics (NCMH), School of Biosciences, University of Nottingham, Sutton Bonington, LE12 5RD, UK.
  • MacCalman TE; National Centre for Macromolecular Hydrodynamics (NCMH), School of Biosciences, University of Nottingham, Sutton Bonington, LE12 5RD, UK.
  • Pattem J; School of Biosciences, University of Nottingham, Sutton Bonington, LE12 5RD, UK.
  • Goodall M; National Centre for Macromolecular Hydrodynamics (NCMH), School of Biosciences, University of Nottingham, Sutton Bonington, LE12 5RD, UK.
  • Gillis RB; School of Biosciences, University of Nottingham, Sutton Bonington, LE12 5RD, UK.
  • Jefferis R; Institute of Immunology & Immunotherapy, College of Medical & Dental Sciences, University of Birmingham, Birmingham, B15 2TT, UK.
  • Harding SE; National Centre for Macromolecular Hydrodynamics (NCMH), School of Biosciences, University of Nottingham, Sutton Bonington, LE12 5RD, UK.
Eur Biophys J ; 52(4-5): 439-443, 2023 Jul.
Article en En | MEDLINE | ID: mdl-37195494
The solution properties of two different glycoforms of IgG1 (IgG1Cri and IgG1Wid) are compared using primarily sedimentation equilibrium analysis with two complementary analysis routines: SEDFIT-MSTAR and MULTISIG. IgGCri bears diantennary complex-type glycans on its Fc domain that are fully core fucosylated and partially sialylated, whilst on IgGWid, they are non-fucosylated, partially galactosylated and non-sialylated. IgGWid is also Fab glycosylated. Despite these differences, SEDFIT-MSTAR analysis shows similar weight average molar masses Mw of ~ (150 ± 5) kDa for IgGCri and ~ (154 ± 5) kDa for IgGWid and both glycoforms show evidence of the presence of a small fraction of dimer confirmed by MULTISIG analysis and also by sedimentation coefficient distributions from supportive sedimentation velocity measurements. The closeness of the sedimentation equilibrium behaviour and sedimentation coefficient distributions with a main peak sedimentation coefficient of ~ 6.4S for both glycoforms at different concentrations suggest that the different glycosylation profiles do not significantly impact on molar mass (molecular weight) nor conformation in solution.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos / Inmunoglobulina G Idioma: En Revista: Eur Biophys J Asunto de la revista: BIOFISICA Año: 2023 Tipo del documento: Article Pais de publicación: Alemania
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polisacáridos / Inmunoglobulina G Idioma: En Revista: Eur Biophys J Asunto de la revista: BIOFISICA Año: 2023 Tipo del documento: Article Pais de publicación: Alemania