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Cell-intrinsic genomic reassortment of pandemic H1N1 2009 and Eurasian avian-like swine influenza viruses results in potentially zoonotic variants.
Ferrando, Verónica A; Friedrich, Marcel E; Gandhi, Shrey; Mellmann, Alexander; Masemann, Dörthe; Christersson, Anmari; Anhlan, Darisuren; Brunotte, Linda; Stoll, Monika; Harder, Timm; Beer, Martin; Boergeling, Yvonne; Ludwig, Stephan.
Afiliación
  • Ferrando VA; Institute of Virology Münster, Westfälische Wilhelms-University, Münster, Germany.
  • Friedrich ME; Institute of Virology Münster, Westfälische Wilhelms-University, Münster, Germany.
  • Gandhi S; Department of Genetic Epidemiology, Institute of Human Genetics, Westfälische Wilhelms-University, Münster, Germany.
  • Mellmann A; Institute of Hygiene Münster, Westfälische Wilhelms-University, Münster, Germany.
  • Masemann D; Institute of Virology Münster, Westfälische Wilhelms-University, Münster, Germany.
  • Christersson A; Institute of Virology Münster, Westfälische Wilhelms-University, Münster, Germany.
  • Anhlan D; Institute of Virology Münster, Westfälische Wilhelms-University, Münster, Germany.
  • Brunotte L; Institute of Virology Münster, Westfälische Wilhelms-University, Münster, Germany.
  • Stoll M; Department of Genetic Epidemiology, Institute of Human Genetics, Westfälische Wilhelms-University, Münster, Germany.
  • Harder T; Institute of Diagnostic Virology, Friedrich-Loeffler-Institute, Greifswald, Germany.
  • Beer M; Institute of Diagnostic Virology, Friedrich-Loeffler-Institute, Greifswald, Germany.
  • Boergeling Y; Institute of Virology Münster, Westfälische Wilhelms-University, Münster, Germany.
  • Ludwig S; Institute of Virology Münster, Westfälische Wilhelms-University, Münster, Germany.
Emerg Microbes Infect ; 12(1): 2212809, 2023 Dec.
Article en En | MEDLINE | ID: mdl-37191590
Influenza A viruses (IAV) cause annual epidemics and occasional pandemics in humans. The most recent pandemic outbreak occurred in 2009 with H1N1pdm09. This virus, which most likely reassorted in swine before its transmission to humans, was reintroduced into the swine population and continues circulating ever since. In order to assess its potential to cause reassortants on a cellular level, human origin H1N1pdm09 and a recent Eurasian avian-like H1N1 swine IAV were (co-)passaged in the newly generated swine lung cell line C22. Co-infection with both viruses gave rise to numerous reassortants that additionally carry different mutations which can partially be found in nature as well. Reassortment most frequently affected the PB1, PA and NA segments with the swine IAV as recipient. These reassortants reached higher titers in swine lung cells and were able to replicate in genuine human lung tissue explants ex vivo, suggesting a possible zoonotic potential. Interestingly, reassortment and mutations in the viral ribonucleoprotein complex influence the viral polymerase activity in a cell type and species-specific manner. In summary, we demonstrate reassortment promiscuity of these viruses in a novel swine lung cell model and indicate a possible zoonotic potential of the reassortants.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus de la Influenza A / Enfermedades de los Porcinos / Infecciones por Orthomyxoviridae / Gripe Humana / Subtipo H1N1 del Virus de la Influenza A Límite: Animals / Humans Idioma: En Revista: Emerg Microbes Infect Año: 2023 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus de la Influenza A / Enfermedades de los Porcinos / Infecciones por Orthomyxoviridae / Gripe Humana / Subtipo H1N1 del Virus de la Influenza A Límite: Animals / Humans Idioma: En Revista: Emerg Microbes Infect Año: 2023 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos