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Cross-sectional analysis of Piroplasma species-infecting camel (Camelus dromedaries) in Egypt using a multipronged molecular diagnostic approach.
Mahdy, Olfat A; Nassar, Ahmed M; Elsawy, Bassma S M; Alzan, Heba F; Kandil, Omnia M; Mahmoud, Mona S; Suarez, Carlos E.
Afiliación
  • Mahdy OA; Parasitology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
  • Nassar AM; Parasitology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
  • Elsawy BSM; Parasitology and Animal Diseases Department, Veterinary Institute, National Research Centre, Giza, Egypt.
  • Alzan HF; Tick and Tick-Borne Diseases Research Unit, National Research Centre, Giza, Egypt.
  • Kandil OM; Parasitology and Animal Diseases Department, Veterinary Institute, National Research Centre, Giza, Egypt.
  • Mahmoud MS; Tick and Tick-Borne Diseases Research Unit, National Research Centre, Giza, Egypt.
  • Suarez CE; Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, WA, United States.
Front Vet Sci ; 10: 1178511, 2023.
Article en En | MEDLINE | ID: mdl-37187929
Camel piroplasmosis is a tick-borne disease (TBD) caused by hemoprotozoan parasites. Hereby, we describe a cross-sectional study aiming at identifying Piroplasma spp.-infecting camels in Egypt using a multipronged molecular diagnostic approach. A total of 531 blood samples from camels (Camelus dromedarius) were collected from slaughterhouses at different governorates in Egypt for analysis during the period from June 2018 to May 2019. Piroplasma spp. was identified using microscopical examination and several different and sequential polymerase chain reaction (PCR) assays targeting the 18S rRNA genes. The overall prevalence of Piroplasma spp. in microscopical and molecular analyses in the samples was 11% (58/531) and 38% (203/531), respectively. Further discriminative multiplex PCR analysis targeting the 18S rRNA gene applied on all Piroplasma spp.-positive samples allowed the detection of Theileria equi (41%), Babesia caballi (5.4%), Babesia bigemina (0.5%), and Babesia bovis (4%). Additionally, the blast analysis of nested (n) PCR, targeting the V4 region, amplicon sequences resulted in the identification of B. vulpes (22%), Babesia sp. (9%), and Theileria sp. (3%). Overall, the results of this study confirmed the high prevalence of TBDs caused by several types of piroplasm hemoparasites in camel and suggests the need for future interventions aimed at improving the control of these potentially debilitating diseases that may be t-hreatening important economic resources and food security in Egypt.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies / Observational_studies / Prevalence_studies / Risk_factors_studies Idioma: En Revista: Front Vet Sci Año: 2023 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Tipo de estudio: Diagnostic_studies / Observational_studies / Prevalence_studies / Risk_factors_studies Idioma: En Revista: Front Vet Sci Año: 2023 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Suiza