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Nitric oxide-mediated S-nitrosylation of IAA17 protein in intrinsically disordered region represses auxin signaling.
Jing, Hongwei; Yang, Xiaolu; Emenecker, Ryan J; Feng, Jian; Zhang, Jian; Figueiredo, Marcelo Rodrigues Alves de; Chaisupa, Patarasuda; Wright, R Clay; Holehouse, Alex S; Strader, Lucia C; Zuo, Jianru.
Afiliación
  • Jing H; State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China; Department of Biology, Duke University, Durham, NC 27008, USA. Electronic address: hongwei.ji
  • Yang X; State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China; Department of Neurobiology, Duke University Medical Center, Durham, NC 27710, USA.
  • Emenecker RJ; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63110, USA; Center for Biomolecular Condensates (CBC), Washington University in St. Louis, St. Louis, MO 63130, USA.
  • Feng J; State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
  • Zhang J; State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China.
  • Figueiredo MRA; Department of Biology, Duke University, Durham, NC 27008, USA.
  • Chaisupa P; Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA 24061, USA.
  • Wright RC; Department of Biological Systems Engineering, Virginia Tech, Blacksburg, VA 24061, USA; The Translational Plant Sciences Center (TPSC), Virginia Tech, Blacksburg, VA 24061, USA.
  • Holehouse AS; Department of Biochemistry and Molecular Biophysics, Washington University School of Medicine, St. Louis, MO 63110, USA; Center for Biomolecular Condensates (CBC), Washington University in St. Louis, St. Louis, MO 63130, USA.
  • Strader LC; Department of Biology, Duke University, Durham, NC 27008, USA.
  • Zuo J; State Key Laboratory of Plant Genomics, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China; CAS Center for Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Beijing 1
J Genet Genomics ; 50(7): 473-485, 2023 07.
Article en En | MEDLINE | ID: mdl-37187411
The phytohormone auxin plays crucial roles in nearly every aspect of plant growth and development. Auxin signaling is activated through the phytohormone-induced proteasomal degradation of the Auxin/INDOLE-3-ACETIC ACID (Aux/IAA) family of transcriptional repressors. Notably, many auxin-modulated physiological processes are also regulated by nitric oxide (NO) that executes its biological effects predominantly through protein S-nitrosylation at specific cysteine residues. However, little is known about the molecular mechanisms in regulating the interactive NO and auxin networks. Here, we show that NO represses auxin signaling by inhibiting IAA17 protein degradation. NO induces the S-nitrosylation of Cys-70 located in the intrinsically disordered region of IAA17, which inhibits the TIR1-IAA17 interaction and consequently the proteasomal degradation of IAA17. The accumulation of a higher level of IAA17 attenuates auxin response. Moreover, an IAA17C70W nitrosomimetic mutation renders the accumulation of a higher level of the mutated protein, thereby causing partial resistance to auxin and defective lateral root development. Taken together, these results suggest that S-nitrosylation of IAA17 at Cys-70 inhibits its interaction with TIR1, thereby negatively regulating auxin signaling. This study provides unique molecular insights into the redox-based auxin signaling in regulating plant growth and development.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arabidopsis / Proteínas de Arabidopsis Idioma: En Revista: J Genet Genomics Año: 2023 Tipo del documento: Article Pais de publicación: China
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Arabidopsis / Proteínas de Arabidopsis Idioma: En Revista: J Genet Genomics Año: 2023 Tipo del documento: Article Pais de publicación: China