Your browser doesn't support javascript.
loading
A highly selective probe for fluorometric sensing of cyanide in an aqueous solution and its application in quantitative determination and living cell imaging.
Satheeshkumar, K; Saravanakumar, P; Kalavathi, A; Vennila, K N; Ciattini, S; Chelazzi, L; Elango, Kuppanagounder P.
Afiliación
  • Satheeshkumar K; Department of Chemistry, Gandhigram Rural Institute (Deemed to be University), Gandhigram 624302, India.
  • Saravanakumar P; Department of Chemistry, Gandhigram Rural Institute (Deemed to be University), Gandhigram 624302, India.
  • Kalavathi A; Department of Chemistry, Gandhigram Rural Institute (Deemed to be University), Gandhigram 624302, India.
  • Vennila KN; Department of Chemistry, Gandhigram Rural Institute (Deemed to be University), Gandhigram 624302, India.
  • Ciattini S; Structural Crystallography Centre, University of Florence, Sesto Fiorentino (Florence), Italy.
  • Chelazzi L; Structural Crystallography Centre, University of Florence, Sesto Fiorentino (Florence), Italy.
  • Elango KP; Department of Chemistry, Gandhigram Rural Institute (Deemed to be University), Gandhigram 624302, India. Electronic address: drkpelango@rediffmail.com.
Methods ; 215: 1-9, 2023 07.
Article en En | MEDLINE | ID: mdl-37187297
A simple fluorescent probe (KS4) containing multiple reaction sites (phenolic -OH, imine and C = C bonds) is successfully synthesized and characterized using 1H NMR, 13C NMR, mass and single crystal XRD techniques. KS4 exhibits high selectivity towards CN- over a wide range of common anions in H2O:DMSO (1:1 v/v) leading to an amazing turn-on fluorescence at 505 nm via deprotonation of the phenolic -OH group. The limit of detection (1.3 µM) for CN- was much below the standard (1.9 µM) set by the World Health Organization (WHO). Stoichiometry of the interaction between KS4 and CN- was ascertained as 1:1 by the Job's plot method and the binding constant was determined to be 1.5x104 M-1. Density Functional Theory (DFT) and Time-Dependent Density Functional Theory (TD-DFT) based theoretical insight has been appealed to understand the optical properties of KS4 before and after the addition of CN- ion. The probe shows respectable real-time applicability for qualitative detection of CN- in almond and cassava powder as well as quantification in real water samples with excellent recoveries (98.8 - 99.8%). In addition, KS4 is found to safe towards living HeLa cells and successfully applied to the detection of endogenous cyanide ions in HeLa cells.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Agua / Cianuros Tipo de estudio: Diagnostic_studies / Qualitative_research Límite: Humans Idioma: En Revista: Methods Asunto de la revista: BIOQUIMICA Año: 2023 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Agua / Cianuros Tipo de estudio: Diagnostic_studies / Qualitative_research Límite: Humans Idioma: En Revista: Methods Asunto de la revista: BIOQUIMICA Año: 2023 Tipo del documento: Article País de afiliación: India Pais de publicación: Estados Unidos